Does it make sense to target one tumor cell chemotactic factor or its receptor when several chemotactic axes are involved in metastasis of the same cancer?
© The Author(s) 2016
Received: 6 July 2016
Accepted: 28 July 2016
Published: 10 August 2016
The major problem with cancer progression and anti-cancer therapy is the inherent ability of cancer cells to migrate and establish distant metastases. This ability to metastasize correlates with the presence in a growing tumor of cells with a more malignant phenotype, which express certain cancer stem cell markers. The propensity of malignant cells to migrate and their resistance to radio-chemotherapy somewhat mimics the properties of normal developmentally early stem cells that migrate during organogenesis in the developing embryo. In the past, several factors, including cell migration-promoting cytokines, chemokines, growth factors, bioactive lipids, extracellular nucleotides, and even H+ ions, were found to influence the metastasis of cancer cells. This plethora of pro-migratory factors demonstrates the existence of significant redundancy in the chemoattractants for cancer cells. In spite of this obvious fact, significant research effort has been dedicated to demonstrating the crucial involvement of particular pro-metastatic factor–receptor axes and the development of new drugs targeting one receptor or one chemoattractant. Based on our own experience working with a model of metastatic rhabdomyosarcoma as well as the work of others, in this review we conclude that targeting a single receptor–ligand pro-metastatic axis will not effectively prevent metastasis and that we should seek other more effective therapeutic options.
The list of candidate metastatic factors for cancer cells is very long and includes cell migration-promoting chemokines (e.g., stromal-derived factor 1, SDF-1), growth factors (e.g., hepatocyte growth factor/scatter factor, HGF/SF), bioactive lipids (e.g., sphingosine-1-phosphate, S1P; ceramide-1-phosphate, C1P), extracellular nucleotides (e.g., ATP, UTP), and even H+ ions [3–10]. The migration of cancer cells may also be affected by certain hormones (e.g., follicle-stimulating hormone, FSH; luteinizing hormone, LH), cleavage fragments of the complement cascade (C3 and C5 cleavage fragments; C3a and C5a, respectively), components of the coagulation cascade (e.g., thrombin), and certain danger-associated molecular pattern molecules (DAMPs; e.g., S100 proteins) [11–16].
Pro-metastatic factors activate various specific, corresponding types of receptors, including cytokine receptors, tyrosine kinase receptors, and G protein-coupled receptors. Signals transduced from these receptors activate similar signaling pathways involved in the regulation of cell migration or adhesion and affect elements of the intracellular cytoskeleton [17–19].
The redundancy of factors and receptors involved in migration of cells in the same type of cancer poses an important question: Is it reasonable to target particular pro-migratory axes when several other pro-metastatic axes exist for a given tumor cell? Moreover, in most of the published reports demonstrating migration, “supraphysiological concentrations” of pro-metastatic factors were employed at doses not encountered in normal tissues and that may not be relevant to clinical situations. In addition, the responsiveness of primary tumor cells may change over time as a malignancy progresses and could be affected by several additional clinical problems that emerge in patients, such as infections or organ failure.
In this review we will summarize several years of experience in identifying and blocking crucial pro-metastatic axes involved in the metastasis of human rhabdomyosarcoma (RMS) cells [6, 12, 20–25]. Our observations, obtained with an RMS cell metastasis model, are also relevant to other types of malignancies, as significant redundancy in pro-metastatic ligand–receptor axes exists for almost all tumor types studied so far.
Rhabdomyosarcoma as a model to study cancer metastasis
Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of adolescence and childhood and reportedly accounts for 5 % of all malignant tumors in patients under 15 years of age . Two major histological subtypes have been described: alveolar rhabdomyosarcoma (ARMS) and embryonal rhabdomyosarcoma (ERMS) . ARMS is associated with more aggressive behavior and a worse prognosis than ERMS . Together with neuroblastoma, nephroblastoma, and Ewing’s sarcoma, RMS belongs in the family of so-called “small round blue tumor cells”, which often infiltrate bone marrow (BM). These tumor cells on BM smears are sometimes misdiagnosed as acute leukemia cells [29, 30].
The two types of RMS show differences at the molecular level. ARMS is characterized by the translocation (2;13)(q35;q14) in 70 % of cases and the variant translocation (1;13)(p36;q14) in a smaller percentage of cases [31, 32]. These translocations disrupt the PAX3 and PAX7 genes on chromosomes 2 and 1, respectively, and the FOXO1 gene on chromosome 13, which leads to the generation of PAX3–FOXO1 and PAX7–FOXO1 fusion genes. PAX3–FOXO1 and PAX7–FOXO1 fusion proteins have enhanced transcriptional activity compared with wild type PAX3 and PAX7 and are postulated to play a role in cell survival and dysregulation of the cell cycle in ARMS . Since there are also ARMS cases that are fusion-negative and have a better outcome than fusion-positive cases, it was more recently recommended that RMS should be classified into fusion-positive (PAX3–FOXO1 and PAX7–FOXO1) and fusion-negative tumors . In our experiments over the past 15 years to study RMS metastasis, we have employed several human RMS cell lines, including both fusion-positive (e.g., RH28, RH30, RH41) and fusion-negative (e.g., JR, RD, RH18, RH36, SMS-CTR) tumor cell lines [8, 20, 21]. Some of our results were subsequently verified in primary RMS patient tissue samples [25, 33].
However a lot of progress has been made to understand pathogenesis of RMS, the origin of cells that gives rise in skeletal muscle tissue to this malignancy is still under debate. It has been proposed that, while low-passage mesenchymal stem cells (MSCs) can generate ARMS, low-passage myoblasts can form ERMS [34–36]. On the other hand, RMS cells express several cancer testis antigens (CTAs), which are characteristic of germline-derived cells [37–41]. This observation makes a somewhat hypothetical connection to a concept presented 150 years ago by Rudolf Virchow  and Julius Conheim , who proposed the “embryonic rest hypothesis of cancer development” . According to this hypothesis, certain malignancies may develop from dormant embryonic or germ cells residing in adult tissues . In this context, small round blue cell tumors, (e.g., RMS) that express several CTA antigens are potential candidates to form such malignancies. This hypothesis, however, requires further study. In any case, RMS cell lines, which are endowed with migratory potential, are a convenient model for studying cancer metastasis.
Assays with which to study the metastasis of cancer cells
By employing this in vitro Transwell assay and the in vivo cancer cell seeding efficiency assay, it is possible, in a relatively easy way, to study the contribution of several potential pro-metastatic factor–receptor axes to cancer metastasis and to test the efficacy of various anti-metastatic strategies [8, 21, 22, 33].
“The never-ending story” of pro-metastatic factors for RMS cells
In the past 15 years we have identified several factors involved in directing the migration of RMS cells and thus potentially directing metastasis of this tumor. The first factors that we studied were cytokines with chemotactic activity, known as chemokines [6, 9, 20–22]. Chemokines regulate the migration of several types of normal cells, activate seven-transmembrane-domain G protein-coupled receptors, and it is not surprising that they also chemoattract cancer cells [18, 23, 36, 46–48]. For example, we demonstrated that SDF-1, by engaging both CXCR4 and CXCR7 seven-transmembrane-domain receptors, promotes migration of RMS cells and could be responsible for their metastasis to BM [6, 22]. Specifically, we showed that RMS cells respond robustly to gradients of SDF-1 employed at high concentrations, and this migration was inhibited by blocking CXCR4 with small-molecule antagonists . Later on, when a new ligand for CXCR4, the chemokine macrophage migration inhibitory factor (MIF), had been described , we also confirmed that it may direct migration of CXCR4+ RMS cells . Since RMS cells express CXCR7, they may also respond to another chemokine, interferon-inducible T cell alpha chemoattractant (I-TAC) . The role of chemokines in regulating the biology of RMS cells is even more complicated, as RMS cells may secrete interleukin 8 (IL-8). Since they do not express the corresponding receptors (CXCR1 and CXCR2), IL-8 secreted by RMS cells exerts paracrine effects on the surrounding microenvironment and stimulates tumor angiogenesis .
RMS cells also respond to several growth factors that engage receptors with intrinsic tyrosine kinase activity [18, 51]. It has been reported that insulin-like growth factor 1 and 2 are not only RMS growth-promoting factors but are also potent chemotactic factors for these cells [18, 52–54]. In our own work we also confirmed that hepatocyte growth factor/scatter factor (HGF/SF) promotes migration and adhesion of RMS cells by engaging the c-Met receptor .
Another group of factors that may direct migration of RMS cells are cytokines, and our recent research demonstrated the involvement of erythropoietin in enhancing the pro-metastatic potential of this tumor . Erythropoietin is very often employed in patients to ameliorate chemotherapy-induced anemia . Therefore, erythropoietin supplementation in RMS patients may have the unwanted side effect of stimulating tumor progression.
In addition to peptide-based factors, such as cytokines, chemokines, and growth factors, another potent class of pro-metastatic factors for RMS cells that we have identified is bioactive lipids. In our recent work we have demonstrated that the pro-metastatic potential of RMS cells is enhanced by the presence of sphingosine-1-phosphate (S1P), ceramide-1-phosphate (C1P), lysophosphatidylcholine (LPC), and its derivative, lysophosphatidic acid (LPA) . All these bioactive lipids strongly enhance motility and adhesion of human RMS cells, and, more importantly, these metastatic-associated phenomena were observed at physiological concentrations of these lipids that naturally occur in biological fluids .
Moreover, a novel class of factors that we identified that may enhance the migration of RMS cells is gonadal and pituitary sex hormones (SexHs) . SexHs are involved in skeletal muscle development and regeneration, and we found that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) receptors are expressed in established human RMS cell lines as well as in primary tumor samples isolated from RMS patients. We also found that human RMS cell lines responded both to pituitary and gonadal SexH stimulation by enhanced proliferation, chemotaxis, and cell adhesion . The expression of functional SexHs by RMS cells suggests, as mentioned above, their developmental relationship with certain developmentally early stem cells deposited in adult tissues [57, 58].
Finally, metastasis and migration of RMS cells are also affected by several other factors, such as extracellular microvesicles (ExMVs) [59, 60], thrombin , and even extracellular nucleotides (e.g., ATP, UTP) . The list of these factors is still open, and new candidates are being identified.
Other strategies to inhibit the metastasis of cancer cells
Based on our results for a model of RMS metastasis, we conclude that there are multiple redundant pro-metastatic axes for this tumor. Therefore inhibition of one of these axes will not prevent pro-metastatic responsiveness of the cells to other axes. Instead, most ideal anti-metastatic treatment should target common mechanisms involved in the metastatic process.
One of these possibilities is to target signaling pathways involved in cell migration such as intracellular kinases that are known to promote this process (e.g., p42/44 MAPK, AKT, or PKC). However, since these signaling kinases are involved in the regulation of many physiological processes, it would be difficult to target them without unpredictable side effects.
Another potential strategy would be to enhance the expression of certain stress-specific pathways that inhibit cell migration. One such strategy that we recently identified is to upregulate heme oxygenase 1 (HO-1) in cancer cells (manuscript in preparation). Small-molecule inducers of this stress-induced enzyme are available, and our preliminary results in several tumor models demonstrate the efficacy of such treatment in inhibiting the spread of cancer cells [61, 62]. However, in parallel we have to take into consideration other potential pleiotropic effects of HO-1 on tumor cells.
Finally, we have proposed that a pro-metastatic microenvironment may be induced in healthy tissues in response to radio-chemotherapy [63, 64]. While there are several well-known side effects of chemotherapy and radiotherapy that are mainly related to toxicity and the impaired function of several organs, the induction of a pro-metastatic microenvironment is still, surprisingly, not widely acknowledged [63, 64]. We proposed that toxic damage in various organs leads to upregulation in “bystander” tissues of several chemotactic factors, which attract circulating stem cells for regeneration but unfortunately also provide chemotactic signals to attract cancer cells that survived the initial treatment . This mechanism may play an important role in the metastasis of cancer cells to organs such as bones, lungs, and liver, which are highly susceptible to chemotherapeutic agents as well as ionizing irradiation. We have demonstrated that this side effect of radio-chemotherapy can be ameliorated by administration of non-steroid anti-inflammatory drugs (e.g., ibuprofen) or steroids at the time of administration of radiochemotherapeutic treatment . This strategy may effectively ameliorate collateral induction of pro-metastatic factors in various organs and tissue. Based on our experimental data these new potential therapeutic strategies shall be tested in the clinical settings.
As mentioned in introduction metastasis is responsible for more than 90 % of cancer-associated mortality and therefore the clinical need to prevent or target metastasis is one of the therapeutic priorities in clinical oncology. Our long-standing studies on the mechanisms involved in cancer metastasis by employing RMS cells as a model system clearly show that targeting a single receptor–ligand axis may slow down but will not prevent cancer cells from undergoing metastasis, as several redundant pro-metastatic receptor–ligand axes exist. Moreover, based on the literature and taking into consideration that multiple pro-metastatic factors have also been identified for other types of malignancies, the general conclusions of this review apply also to other tumors. Therefore, there is an urgent need to develop more efficient anti-metastatic therapies that will simultaneously target the response of cancer cells to all pro-metastatic factors (e.g., by intracellular upregulation of HO-1) or even to employ as a prophylactic treatment drugs (e.g., steroids or anti-inflammatory drugs) that prevent induction of a pro-metastatic microenvironment induced in various organs after radio-chemotherapy. These new potential anti-metastatic strategies could be combined with surgical treatment and/or cancer immunotherapy. A variety of novel surgical approaches as well as strategies to stimulate the immune system to destroy growing tumors are available including T-cell adoptive transfer combined with inteleukin-2 therapy, genetically engineered T cells specialized to recognize tumor antigens or autologous immune enhancement therapy using patient’s own peripheral blood-derived NK cells or other relevant immune cells [65–67].
MZR wrote manuscript, MS design figures. All authors read and approved the final manuscript.
This work was supported by NIH Grants 2R01 DK074720 and R01HL112788, the Stella and Henry Endowment, and Harmonia NCN Grant: UMO-2014/14/M/NZ3/00475 to MZR.
The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Steeg PS (2006) Tumor metastasis: mechanistic insights and clinical challenges. Nat Med 12:895–904View ArticlePubMedGoogle Scholar
- Petrie RJ, Doyle AD, Yamada KM (2009) Random versus directionally persistent cell migration. Nat Rev Mol Cell Biol 10:538–549View ArticlePubMedPubMed CentralGoogle Scholar
- Ferracini R, Olivero M, Di Renzo MF, Martano M, De Giovanni C, Nanni P, Basso G, Scotlandi K, Lollini PL, Comoglio PM (1996) Retrogenic expression of the MET proto-oncogene correlates with the invasive phenotype of human rhabdomyosarcomas. Oncogene 12:1697–1705PubMedGoogle Scholar
- Geminder H, Sagi-Assif O, Goldberg L, Meshel T, Rechavi G, Witz IP, Ben-Baruch A (2001) A possible role for CXCR4 and its ligand, the CXC chemokine stromal cell-derived factor-1, in the development of bone marrow metastases in neuroblastoma. J Immunol 167:4747–4757View ArticlePubMedGoogle Scholar
- Muller A, Homey B, Soto H, Ge N, Catron D, Buchanan ME, McClanahan T, Murphy E, Yuan W, Wagner SN, Barrera JL, Mohar A, Verastegui E, Zlotnik A (2001) Involvement of chemokine receptors in breast cancer metastasis. Nature 410:50–56View ArticlePubMedGoogle Scholar
- Libura J, Drukala J, Majka M, Tomescu O, Navenot JM, Kucia M, Marquez L, Peiper SC, Barr FG, Janowska-Wieczorek A, Ratajczak MZ (2002) CXCR4-SDF-1 signaling is active in rhabdomyosarcoma cells and regulates locomotion, chemotaxis, and adhesion. Blood 100:2597–2606View ArticlePubMedGoogle Scholar
- Davicioni E, Anderson MJ, Finckenstein FG, Lynch JC, Qualman SJ, Shimada H, Schofield DE, Buckley JD, Meyer WH, Sorensen PH, Triche TJ (2009) Molecular classification of rhabdomyosarcoma—genotypic and phenotypic determinants of diagnosis: a report from the Children’s Oncology Group. Am J Pathol 174:550–564View ArticlePubMedPubMed CentralGoogle Scholar
- Schneider G, Bryndza E, Abdel-Latif A, Ratajczak J, Maj M, Tarnowski M, Klyachkin YM, Houghton P, Morris AJ, Vater A, Klussmann S, Kucia M, Ratajczak MZ (2013) Bioactive lipids S1P and C1P are prometastatic factors in human rhabdomyosarcoma, and their tissue levels increase in response to radio/chemotherapy. Mol Cancer Res 11:793–807View ArticlePubMedPubMed CentralGoogle Scholar
- Wysoczynski M, Miekus K, Jankowski K, Wanzeck J, Bertolone S, Janowska-Wieczorek A, Ratajczak J, Ratajczak MZ (2007) Leukemia inhibitory factor: a newly identified metastatic factor in rhabdomyosarcomas. Cancer Res 67:2131–2140View ArticlePubMedGoogle Scholar
- Damaghi M, Wojtkowiak JW, Gillies RJ (2013) pH sensing and regulation in cancer. Front Physiol 4:370View ArticlePubMedPubMed CentralGoogle Scholar
- Abdelbaset-Ismail A, Borkowska S, Janowska-Wieczorek A, Tonn T, Rodriguez C, Moniuszko M, Bolkun L, Koloczko J, Eljaszewicz A, Ratajczak J, Ratajczak MZ, Kucia M (2016) Novel evidence that pituitary gonadotropins directly stimulate human leukemic cells-studies of myeloid cell lines and primary patient AML and CML cells. Oncotarget 7:3033–3046PubMedGoogle Scholar
- Wysoczynski M, Liu R, Kucia M, Drukala J, Ratajczak MZ (2010) Thrombin regulates the metastatic potential of human rhabdomyosarcoma cells: distinct role of PAR1 and PAR3 signaling. Mol Cancer Res 8:677–690View ArticlePubMedPubMed CentralGoogle Scholar
- Krysko O, Love Aaes T, Bachert C, Vandenabeele P, Krysko DV (2013) Many faces of DAMPs in cancer therapy. Cell Death Dis 4:e631View ArticlePubMedPubMed CentralGoogle Scholar
- Al-Rayahi IA, Sanyi RH (2015) The overlapping roles of antimicrobial peptides and complement in recruitment and activation of tumor-associated inflammatory cells. Front Immunol 6:2View ArticlePubMedPubMed CentralGoogle Scholar
- Rutkowski MJ, Sughrue ME, Kane AJ, Mills SA, Parsa AT (2010) Cancer and the complement cascade. Mol Cancer Res 8:1453–1465View ArticlePubMedGoogle Scholar
- Surace L, Lysenko V, Fontana AO, Cecconi V, Janssen H, Bicvic A, Okoniewski M, Pruschy M, Dummer R, Neefjes J, Knuth A, Avan Gupta, den Broek M (2015) Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response. Immunity 42:767–777View ArticlePubMedGoogle Scholar
- Wang J, Knaut H (2014) Chemokine signaling in development and disease. Development 141:4199–4205View ArticlePubMedPubMed CentralGoogle Scholar
- Comoglio PM, Trusolino L (2002) Invasive growth: from development to metastasis. J Clin Invest 109:857–862View ArticlePubMedPubMed CentralGoogle Scholar
- Forgacs G, Yook SH, Janmey PA, Jeong H, Burd CG (2004) Role of the cytoskeleton in signaling networks. J Cell Sci 117:2769–2775View ArticlePubMedGoogle Scholar
- Jankowski K, Kucia M, Wysoczynski M, Reca R, Zhao D, Trzyna E, Trent J, Peiper S, Zembala M, Ratajczak J, Houghton P, Janowska-Wieczorek A, Ratajczak MZ (2003) Both hepatocyte growth factor (HGF) and stromal-derived factor-1 regulate the metastatic behavior of human rhabdomyosarcoma cells, but only HGF enhances their resistance to radiochemotherapy. Cancer Res 63:7926–7935PubMedGoogle Scholar
- Tarnowski M, Grymula K, Liu R, Tarnowska J, Drukala J, Ratajczak J, Mitchell RA, Ratajczak MZ, Kucia M (2010) Macrophage migration inhibitory factor is secreted by rhabdomyosarcoma cells, modulates tumor metastasis by binding to CXCR4 and CXCR7 receptors and inhibits recruitment of cancer-associated fibroblasts. Mol Cancer Res 8:1328–1343View ArticlePubMedPubMed CentralGoogle Scholar
- Grymula K, Tarnowski M, Wysoczynski M, Drukala J, Barr FG, Ratajczak J, Kucia M, Ratajczak MZ (2010) Overlapping and distinct role of CXCR7-SDF-1/ITAC and CXCR4-SDF-1 axes in regulating metastatic behavior of human rhabdomyosarcomas. Int J Cancer 127:2554–2568View ArticlePubMedPubMed CentralGoogle Scholar
- Maksym RB, Tarnowski M, Grymula K, Tarnowska J, Wysoczynski M, Liu R, Czerny B, Ratajczak J, Kucia M, Ratajczak MZ (2009) The role of stromal-derived factor-1–CXCR7 axis in development and cancer. Eur J Pharmacol 625:31–40View ArticlePubMedPubMed CentralGoogle Scholar
- Schneider G, Glaser T, Lameu C, Abdelbaset-Ismail A, Sellers ZP, Moniuszko M, Ulrich H, Ratajczak MZ (2015) Extracellular nucleotides as novel, underappreciated pro-metastatic factors that stimulate purinergic signaling in human lung cancer cells. Mol Cancer 14:201View ArticlePubMedPubMed CentralGoogle Scholar
- Schneider G, Sellers ZP, Abdel-Latif A, Morris AJ, Ratajczak MZ (2014) Bioactive lipids, LPC and LPA, are novel prometastatic factors and their tissue levels increase in response to radio/chemotherapy. Mol Cancer Res 12:1560–1573View ArticlePubMedPubMed CentralGoogle Scholar
- Ferrari A, Bisogno G, Macaluso A, Casanova M, D’Angelo P, Pierani P, Zanetti I, Alaggio R, Cecchetto G, Carli M (2007) Soft-tissue sarcomas in children and adolescents with neurofibromatosis type 1. Cancer 109:1406–1412View ArticlePubMedGoogle Scholar
- Mercado GE, Barr FG (2007) Fusions involving PAX and FOX genes in the molecular pathogenesis of alveolar rhabdomyosarcoma: recent advances. Curr Mol Med 7:47–61View ArticlePubMedGoogle Scholar
- Collins MH, Zhao H, Womer RB, Barr FG (2001) Proliferative and apoptotic differences between alveolar rhabdomyosarcoma subtypes: a comparative study of tumors containing PAX3–FKHR or PAX7–FKHR gene fusions. Med Pediatr Oncol 37(83–89):28Google Scholar
- Sandberg AA, Stone JF, Czarnecki L, Cohen JD (2001) Hematologic masquerade of rhabdomyosarcoma. Am J Hematol 68:51–57View ArticlePubMedGoogle Scholar
- Karagiannis P, Guth N, Thoennissen GB, Bern C, Sperveslage J, Oschlies I, Bokemeyer C, Klapper W, Wardelmann E, Thoennissen NH (2015) Alveolar rhabdomyosarcoma confined to the bone marrow with no identifiable primary tumour using FDG-PET/CT. Clin Sarcoma Res 5:24View ArticlePubMedPubMed CentralGoogle Scholar
- Davis RJ, D’Cruz CM, Lovell MA, Biegel JA, Barr FG (1994) Fusion of PAX7 to FKHR by the variant t(1;13)(p36;q14) translocation in alveolar rhabdomyosarcoma. Cancer Res 54:2869–2872PubMedGoogle Scholar
- Davicioni E, Finckenstein FG, Shahbazian V, Buckley JD, Triche TJ, Anderson MJ (2006) Identification of a PAX-FKHR gene expression signature that defines molecular classes and determines the prognosis of alveolar rhabdomyosarcomas. Cancer Res 66:6936–6946View ArticlePubMedGoogle Scholar
- Tarnowski M, Schneider G, Amann G, Clark G, Houghton P, Barr FG, Kenner L, Ratajczak MZ, Kucia M (2012) RasGRF1 regulates proliferation and metastatic behavior of human alveolar rhabdomyosarcomas. Int J Oncol 41:995–1004PubMedPubMed CentralGoogle Scholar
- Charytonowicz E, Cordon-Cardo C, Matushansky I, Ziman M (2009) Alveolar rhabdomyosarcoma: is the cell of origin a mesenchymal stem cell? Cancer Lett 279:126–136View ArticlePubMedGoogle Scholar
- Hettmer S, Wagers AJ (2010) Muscling in: uncovering the origins of rhabdomyosarcoma. Nat Med 16:171–173View ArticlePubMedGoogle Scholar
- Hettmer S, Liu J, Miller CM, Lindsay MC, Sparks CA, Guertin DA, Bronson RT, Langenau DM, Wagers AJ (2011) Sarcomas induced in discrete subsets of prospectively isolated skeletal muscle cells. Proc Natl Acad Sci U S A 108:20002–20007View ArticlePubMedPubMed CentralGoogle Scholar
- Simpson AJ, Caballero OL, Jungbluth A, Chen YT, Old LJ (2005) Cancer/testis antigens, gametogenesis and cancer. Nat Rev Cancer 5:615–625View ArticlePubMedGoogle Scholar
- Mahlendorf DE, Staege MS (2013) Characterization of Ewing sarcoma associated cancer/testis antigens. Cancer Biol Ther 14:254–261View ArticlePubMedPubMed CentralGoogle Scholar
- Oosterhuis JW, Looijenga LH (2005) Testicular germ-cell tumours in a broader perspective. Nat Rev Cancer 5:210–222View ArticlePubMedGoogle Scholar
- Jacobs JF, Brasseur F, Hulsbergen-van de Kaa CA, van de Rakt MW, Figdor CG, Adema GJ, Hoogerbrugge PM, Coulie PG, de Vries IJ (2007) Cancer-germline gene expression in pediatric solid tumors using quantitative real-time PCR. Int J Cancer 120:67–74View ArticlePubMedGoogle Scholar
- Fratta E, Coral S, Covre A, Parisi G, Colizzi F, Danielli R, Nicolay HJ, Sigalotti L, Maio M (2011) The biology of cancer testis antigens: putative function, regulation and therapeutic potential. Mol Oncol 5:164–182View ArticlePubMedGoogle Scholar
- Virchow R (1855) Archiv fuer pathologische anatomie und physiologie und fuer klinische. Medizin 8:23–54Google Scholar
- Conheim J (1875) Congenitales, quergestreiftes Muskelsarkon der Nireren. Virchows Arch 65:64–69View ArticleGoogle Scholar
- Sell S (2004) Stem cell origin of cancer and differentiation therapy. Crit Rev Oncol Hematol 51:1–28View ArticlePubMedGoogle Scholar
- Schneider G, Sellers ZP, Ratajczak MZ (2016) Induction of a tumor-metastasis-receptive microenvironment as an unwanted side effect after radio/chemotherapy and in vitro and in vivo assays to study this phenomenon. Methods Mol Biol. doi:10.1007/7651_2016_323 Google Scholar
- Avecilla ST, Hattori K, Heissig B, Tejada R, Liao F, Shido K, Jin DK, Dias S, Zhang F, Hartman TE, Hackett NR, Crystal RG, Witte L, Hicklin DJ, Bohlen P, Eaton D, Lyden D, de Sauvage F, Rafii S (2004) Chemokine-mediated interaction of hematopoietic progenitors with the bone marrow vascular niche is required for thrombopoiesis. Nat Med 10:64–71View ArticlePubMedGoogle Scholar
- Boccaccio C, Comoglio PM (2006) Invasive growth: a MET-driven genetic programme for cancer and stem cells. Nat Rev Cancer 6:637–645View ArticlePubMedGoogle Scholar
- Crane IJ, Wallace CA, McKillop-Smith S, Forrester JV (2000) CXCR4 receptor expression on human retinal pigment epithelial cells from the blood–retina barrier leads to chemokine secretion and migration in response to stromal cell-derived factor 1 alpha. J Immunol 165:4372–4378View ArticlePubMedGoogle Scholar
- Bernhagen J, Krohn R, Lue H, Gregory JL, Zernecke A, Koenen RR, Dewor M, Georgiev I, Schober A, Leng L, Kooistra T, Fingerle-Rowson G, Ghezzi P, Kleemann R, McColl SR, Bucala R, Hickey MJ, Weber C (2007) MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment. Nat Med 13:587–596View ArticlePubMedGoogle Scholar
- Wysoczynski M, Shin DM, Kucia M, Ratajczak MZ (2010) Selective upregulation of interleukin-8 by human rhabdomyosarcomas in response to hypoxia: therapeutic implications. Int J Cancer 126:371–381View ArticlePubMedPubMed CentralGoogle Scholar
- Ginsberg JP, Davis RJ, Bennicelli JL, Nauta LE, Barr FG (1998) Up-regulation of MET but not neural cell adhesion molecule expression by the PAX3-FKHR fusion protein in alveolar rhabdomyosarcoma. Cancer Res 58:3542–3546PubMedGoogle Scholar
- Makawita S, Ho M, Durbin AD, Thorner PS, Malkin D, Somers GR (2009) Expression of insulin-like growth factor pathway proteins in rhabdomyosarcoma: IGF-2 expression is associated with translocation-negative tumors. Pediatr Dev Pathol 12:127–135View ArticlePubMedGoogle Scholar
- Rikhof B, de Jong S, Suurmeijer AJ, Cvan Meijer, der Graaf WT (2009) The insulin-like growth factor system and sarcomas. J Pathol 217:469–482View ArticlePubMedGoogle Scholar
- Hahn H, Wojnowski L, Specht K, Kappler R, Calzada-Wack J, Potter D, Zimmer A, Muller U, Samson E, Quintanilla-Martinez L (2000) Patched target Igf2 is indispensable for the formation of medulloblastoma and rhabdomyosarcoma. J Biol Chem 275:28341–28344View ArticlePubMedGoogle Scholar
- Poniewierska-Baran A, Suszynska M, Sun W, Abdelbaset-Ismail A, Schneider G, Barr FG, Ratajczak MZ (2015) Human rhabdomyosarcoma cells express functional erythropoietin receptor: potential therapeutic implications. Int J Oncol 47:1989–1997PubMedPubMed CentralGoogle Scholar
- Broxmeyer HE (2013) Erythropoietin: multiple targets, actions, and modifying influences for biological and clinical consideration. J Exp Med 210:205–208View ArticlePubMedPubMed CentralGoogle Scholar
- Ratajczak MZ, Shin DM, Liu R, Marlicz W, Tarnowski M, Ratajczak J, Kucia M (2010) Epiblast/germ line hypothesis of cancer development revisited: lesson from the presence of Oct-4+ cells in adult tissues. Stem Cell Rev 6:307–316View ArticlePubMedPubMed CentralGoogle Scholar
- Ratajczak MZ, Shin DM, Kucia M (2009) Very small embryonic/epiblast-like stem cells: a missing link to support the germ line hypothesis of cancer development? Am J Pathol 174:1985–1992View ArticlePubMedPubMed CentralGoogle Scholar
- Janowska-Wieczorek A, Marquez-Curtis LA, Wysoczynski M, Ratajczak MZ (2006) Enhancing effect of platelet-derived microvesicles on the invasive potential of breast cancer cells. Transfusion 46:1199–1209View ArticlePubMedGoogle Scholar
- Janowska-Wieczorek A, Wysoczynski M, Kijowski J, Marquez-Curtis L, Machalinski B, Ratajczak J, Ratajczak MZ (2005) Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer. Int J Cancer 113:752–760View ArticlePubMedGoogle Scholar
- Adamiak M, Iv JB, Zhao J, Abdelbaset-Ismail A, Grubczak K, Borkowska S, Wysoczynski M, Ratajczak MZ (2015) Downregulation of heme oxygenase 1 (HO-1) activity in hematopoietic cells enhances their engraftment after transplantation. Cell Transplant 25:1265–1276View ArticleGoogle Scholar
- Wysoczynski M, Ratajczak J, Pedziwiatr D, Rokosh G, Bolli R, Ratajczak MZ (2015) Identification of heme oxygenase 1 (HO-1) as a novel negative regulator of mobilization of hematopoietic stem/progenitor cells. Stem Cell Rev 11:110–118View ArticlePubMedGoogle Scholar
- Gunjal PM, Schneider G, Ismail AA, Kakar SS, Kucia M, Ratajczak MZ (2015) Evidence for induction of a tumor metastasis-receptive microenvironment for ovarian cancer cells in bone marrow and other organs as an unwanted and underestimated side effect of chemotherapy/radiotherapy. J Ovarian Res 8:20View ArticlePubMedPubMed CentralGoogle Scholar
- Ratajczak MZ, Jadczyk T, Schneider G, Kakar S, SKucia M (2013) Induction of a tumor-metastasis-receptive microenvironment as an unwanted and underestimated side effect of treatment by chemotherapy or radiotherapy. J Ovarian Res 6:95View ArticlePubMedPubMed CentralGoogle Scholar
- Maude SL, Teachey DT, Porter DL, Grupp SA (2015) CD19-targeted chimeric antigen receptor T-cell therapy for acute lymphoblastic leukemia. Blood 125:4017–4023View ArticlePubMedPubMed CentralGoogle Scholar
- Posey AD Jr, Schwab RD, Boesteanu AC, Steentoft C, Mandel U, Engels B, Stone JD, Madsen TD, Schreiber K, Haines KM, Cogdill AP, Chen TJ, Song D, Scholler J, Kranz DM, Feldman MD, Young R, Keith B, Schreiber H, Clausen H, Johnson LA, June CH (2016) Engineered CAR T Cells targeting the cancer-associated Tn-glycoform of the membrane mucin MUC1 control adenocarcinoma. Immunity 44:1444–1454View ArticlePubMedGoogle Scholar
- Turtle CJ, Hanafi LA, Berger C, Gooley TA, Cherian S, Hudecek M, Sommermeyer D, Melville K, Pender B, Budiarto TM, Robinson E, Steevens NN, Chaney C, Soma L, Chen X, Yeung C, Wood B, Li D, Cao J, Heimfeld S, Jensen MC, Riddell SR, Maloney DG (2016) CD19 CAR-T cells of defined CD4+:CD8+ composition in adult B cell ALL patients. J Clin Invest 126:2123–2138View ArticlePubMedGoogle Scholar