Fig. 2

In vitro Transwell migration assay. Cells to be tested are placed in the upper chamber, and the migration-promoting factor to be tested for chemotaxis is placed in the lower chamber. If the factor is to be tested for chemokinesis, it is added at the same time to both upper and lower chambers. Cells that migrate to the lower chamber are counted and compared with cells that had migrated in medium without the pro-migratory factor (the control Transwell inserts)