Oncolytic viruses and checkpoint inhibitors: combination therapy in clinical trials
Clinical and Translational Medicine volume 7, Article number: 35 (2018)
Advances in the understanding of cancer immunotherapy and the development of multiple checkpoint inhibitors have dramatically changed the current landscape of cancer treatment. Recent large-scale phase III trials (e.g. PHOCUS, OPTiM) are establishing use of oncolytic viruses as another tool in the cancer therapeutics armamentarium. These viruses do not simply lyse cells to achieve their cancer-killing effects, but also cause dramatic changes in the tumor immune microenvironment. This review will highlight the major vector platforms that are currently in development (including adenoviruses, reoviruses, vaccinia viruses, herpesviruses, and coxsackieviruses) and how they are combined with checkpoint inhibitors. These vectors employ a variety of engineered capsid modifications to enhance infectivity, genome deletions or promoter elements to confer selective replication, and encode a variety of transgenes to enhance anti-tumor or immunogenic effects. Pre-clinical and clinical data have shown that oncolytic vectors can induce anti-tumor immunity and markedly increase immune cell infiltration (including cytotoxic CD8+ T cells) into the local tumor microenvironment. This “priming” by the viral infection can change a ‘cold’ tumor microenvironment into a ‘hot’ one with the influx of a multitude of immune cells and cytokines. This alteration sets the stage for subsequent checkpoint inhibitor delivery, as they are most effective in an environment with a large lymphocytic infiltrate. There are multiple ongoing clinical trials that are currently combining oncolytic viruses with checkpoint inhibitors (e.g. CAPTIVE, CAPRA, and Masterkey-265), and the initial results are encouraging. It is clear that oncolytic viruses and checkpoint inhibitors will continue to evolve together as a combination therapy for multiple types of cancers.
Immunotherapy is at the forefront of cancer research and treatment with the American Society of Clinical Oncology (ASCO) naming immunotherapy as the advance of the year in both 2016 and 2017 [1, 2] and specifically citing adoptive cell immunotherapy as this year’s most important advancement . The large number of clinical trials currently employing immunotherapeutic agents is a testament to the monumental advances they are making in cancer treatment.
Individual immunotherapies have demonstrated remarkable treatment effects in melanoma, lung cancer, and multiple intra-abdominal malignancies . In particular, a class of drugs known as checkpoint inhibitors has been of great interest to researchers and clinicians (Table 1). These antibodies block the negative regulators of T cell function (immune checkpoints), thereby increasing T-cell activation [4, 5]. The United States Food and Drug Administration (FDA) first approved ipilimumab (a monoclonal antibody inhibiting cytotoxic T lymphocyte-associated antigen-4 [CTLA-4]) for the treatment of metastatic melanoma in 2011 . A few years later in 2014, the FDA approved nivolumab and pembrolizumab (both monoclonal antibodies targeting programmed death receptor 1 [PD-1]) for the treatment of advanced melanoma .
The principle of combining therapeutics with complementary mechanisms has also been applied to checkpoint inhibition across a range of malignancies including gastrointestinal and soft tissue cancers [4, 7]. For instance, the Checkmate 067 trial demonstrated the effect of the combination of ipilimumab and nivolumab in patients with untreated melanoma . In these patients with advanced disease, the combination of these two agents resulted in a vast overall survival improvement at 3 years compared with ipilimumab alone (58% vs 34%) [8, 9].
The rapidly advancing field of clinical oncolytic virotherapy is itself coming to be understood as a unique type of immunotherapy. Oncolytic viruses are naturally occurring or genetically modified viruses that infect, replicate in, and kill cancer cells without harming normal cells . Recent decades have seen dramatic advances in gene manipulation capabilities and thus improvements in vector design . Additionally, the understanding of how an oncolytic adenovirus alters the local tumor microenvironment (TME) has led some to think of the field as ‘oncolytic immunotherapy’. Following viral infection, there are increased levels of local cytokine expression as well as an influx of immune cells including natural killer (NK) cells, activated T cells, and antigen presenting cells (APC) . Furthermore, PD-L1 expression is known to increase on tumor and immune cells following viral infection . Taken together, these changes alter the local TME and change it from ‘cold’ to ‘hot’ with a flood of cytokines and immune effectors.
Checkpoint inhibition works best when there is a large lymphocytic infiltrate, which is not always the case for a given tumor [13, 14]. The changes to the local TME following oncolytic virus delivery creates a situation that can be exploited with novel combination regimens, namely oncolytic vectors and checkpoint inhibitors. The efficacy of combining oncolytic viruses and checkpoint inhibition has been shown in pre-clinical data, and there are currently more than 15 ongoing clinical trials employing a combination regimen of these two types of cancer therapeutics (Table 2). With the abundance of ongoing pre-clinical and clinical studies, it is certain that the futures of viral oncolysis and checkpoint inhibition will be intertwined.
In this review, we will explore the combination of oncolytic virotherapy with checkpoint inhibitors. There are many different vector platforms under investigation, all of which are in different stages of development. We will look at a sampling of pre-clinical data, published human trials, and highlight important ongoing clinical trials.
Current-generation oncolytic viral vectors can be engineered to target specific types of cancer cells, selectively replicate within them, and locally express a transgene . The tumor tropism of a virus is a key property that is essential to maximize cancer-killing effects on the tumor, while minimizing the damage to surrounding normal tissues. One approach to optimizing vector replication selectivity is via a tumor-specific or tissue-specific promoter element that is incorporated into the viral genome. Prostate-specific antigen (PSA), cyclooxygenase-2 (Cox2), and human telomerase reverse transcriptase (TERT) promoters are just a few examples of promoter sequences that can be used to confer selective viral replication to target tissues [15,16,17,18,19]. Additionally, deletions in key portions of the viral genome can also allow for selective viral replication. For example, deletions in the E1 region of the adenoviral genome can cause oncolytic adenoviruses to not replicate in normal cells due to the absence of key viral protein products . Then, there must be additional pathway or signaling alterations to facilitate oncolytic virus replication in tumors. One example in many cancers is a defect in the Retinoblastoma (RB)-E2F pathway, which ultimately allows a virus to replicate as its normally negative regulatory function is absent . Also, regulatory proteins from certain cancers (such as the human papilloma virus E6 and E7 oncoproteins) can functionally transcomplement missing viral proteins (such those in an adenovirus with E1 deletions) to allow for viral replication [21, 22]. No matter the mechanism, a key component of any well-designed vector is the ability to selectively replicate in target cells of interest.
As gene therapy approaches have improved, researchers have been able to insert numerous transgenes into multiple different vector platforms to achieve a variety of effects. The size of the viral genome affects the transgene capacity, which makes certain vector systems with larger genomes more desirable. Genes encoding interferon alpha, granulocyte macrophage colony stimulating factor (GM-CSF), and multiple cytokines have all been used as transgenes in oncolytic vectors [12, 23,24,25]. In addition, the sodium iodide symporter (NIS) gene has been used to allow for monitoring of viral replication [26, 27]. The ability to tailor transgene-insertion to the unique purposes of an individual vector makes oncolytic virotherapy a versatile tool in the cancer treatment armamentarium.
Following viral inoculation (especially through an intravenous route), circulating antibodies and the complement cascade can negate the efficacy of an oncolytic virus . As many patients have previously encountered viruses due to vaccination or environmental exposure, it is no surprise that there is a high incidence of neutralizing antibodies to some oncolytic viruses . These effects are often more pronounced following the second and subsequent doses of virus which may serve as a boost to the existing immune response; consequently, researchers have developed multiple approaches to minimize virus neutralization . Strategies include using alternate serotypes, shielding the virus by PEGylation of the viral coat or polymer coating, suppression of the host immune system, and using a carrier (such as mesenchymal stem cells) to deliver the virus to the tumor bed [11, 16, 29,30,31].
Improved infection efficiency, highly-selective replication, and transgene expression make modern-day oncolytic viruses a robust cancer therapeutic that are readily adaptable to combination therapy with other anti-cancer agents.
The immune response and checkpoint inhibitors
The lytic effects of an oncolytic virus only represent a portion of virally-induced cancer-killing potential. Another main component of viral-mediated killing results from the vector’s interactions with the immune system. Immunogenic cell death (ICD) can occur through multiple different mechanisms including autophagy, necrosis, and apoptosis with each of these inducing a different degree and type of immune response , but none of these are adequate to fully characterize the complex interactions that result in oncolytic virus-mediated cell death .
Virus-induced oncolysis can cause the dying cancer cell to release damage associated molecular patterns (DAMPs). These entities (which include cell surface proteins, membrane proteins, and nucleic acids) are released following cell death and can serve as ‘danger signals’ to prime the immune system . Pathogen-associated molecular patterns (PAMP) are produced by various types of microorganisms (including viruses) and are ultimately recognized by pattern recognition receptors (PRRs) in the innate immune system . These ‘danger signals’ are then picked up by antigen presenting cells (APC) such as dendritic cells (DC) and presented to T cells, which then can potentially initiate a systemic, adaptive immune response .
The activation of T cells is a multi-step process which begins with the major histocompatibility complex (MHC) on antigen presenting cells (APC) displaying antigens for recognition by T cell receptors. To achieve T cell activation, there must be a costimulatory signal in the form of B7 molecules on the APC surface binding to CD28 molecules on the T cell surface (Fig. 1a) . CTLA-4 is a member of the immunoglobulin superfamily and is an inhibitory molecule expressed on the surface of activated T cells . It competitively inhibits the binding of B7 to CD28 and effectively diminishes the degree of T cell activation and proliferation (Fig. 1b) . Ipilimumab is one example of a monoclonal antibody that inhibits CTLA-4 and thereby increases T cell activation. PD-1 is a member of the immunoglobulin superfamily present on a variety of immune cells including activated T cells, B cells, NK cells, and antigen presenting cells . Cytokines resulting from infection or tumor formation can induce the release of programmed death receptor ligand 1 (PD-L1), which negatively affects the function of T cells and B cells (Figs. 1c, 2A) . Pembrolizumab is an example of an anti-PD-1 antibody which modulates the PD-1/PD-L1 axis to decrease the negative regulation on lymphocyte activation (Fig. 1d) [6, 13].
The TME is a complex milieu of inflammatory and immune cells that creates an environment which facilitates tumor growth . As discussed previously, immunologically ‘cold’ tumors have a paucity of local infiltrating immune cells, while ‘hot’ tumors have a plethora of circulating immune cells . This is important as immunologically ‘hot’ tumors are known to be more responsive to therapy with checkpoint inhibitors . Therapeutics that can induce a migration of immune cells into the TME can be combined with checkpoint inhibitors to achieve an enhanced effect, and oncolytic viruses have thus far shown much promise in this regard (Fig. 2B, C) [41, 42].
Oncolytic viruses in combination with checkpoint inhibitors
Defining characteristics of the Herpesviridae family include a linear, double-stranded DNA genome, an icosahedral capsid, and a glycoprotein envelope . These are large viruses that can be up to 200 nm in size with a genome that is approximately 150 kb . Importantly, while viral replication occurs in the host cell nucleus, it does not cause insertional mutagenesis as it does not insert into the host genome . There are eight members of this family that commonly infect humans, but Human herpesvirus 1 (a member of the alpha subfamily and more commonly known as herpes simplex virus type 1 [HSV-1]) has been the most studied and tested as a backbone for oncolytic vectors .
Talimogene laherparepvec (T-Vec)
The first FDA-approved oncolytic vector for use in the United States was talimogene laherparepvec (T-Vec). It has a HSV-1 backbone that is modified with deletions in ICP34.5 to augment the tumor selective replication of the virus . T-Vec was also modified with deletions in the ICP47 gene to decrease neurovirulence and inclusion of the human GM-CSF transgene to augment the immune response via improved antigen presentation and T-cell priming . Phase I studies of T-Vec demonstrated it to be a well-tolerated agent. For HSV-seronegative patients, the maximum tolerated single, intratumoral dose was 107 plaque forming unit (pfu)/mL as this titer caused extensive local reactions of inflammation and erythema at the injection site . Notably, HSV-seropositive patients had much less of a local cutaneous reaction. For a multi-injection cohort, seronegative patients were pre-treated with 106 pfu/mL to seroconvert and then treated with two doses of either 107 or 108 pfu/mL with minimal cutaneous reactions . Additionally, there were no obvious differences in clinical response between HSV-seropositive and HSV-seronegative patients in this study . In the phase III OPTiM trial, injection of intralesional T-Vec demonstrated a statistically significant improvement in durable overall response rate when compared to GM-CSF alone (16.2% vs 2.1%, p < 0.001) in patients with unresectable stage IIIB or IV melanoma . Additionally, 15% of measureable visceral (uninjected) lesions reduced in size by 50% or more following treatment with T-Vec . Importantly, intralesional injection with T-Vec into metastatic melanoma lesions alters the immune cell makeup of the tumor microenvironment as demonstrated by a decrease in multiple suppressor cell populations including CD4+ Tregs, CD8+ T suppressor cells, and myeloid derived suppressor cells (MDSC) . Finally, T-Vec-induced local and systemic Melanoma Antigen Recognized by T cells (MART)-1-specific CD8+ effector cells, which suggests the establishment of anti-tumor immunity .
Given the distinct mechanisms of action of T-Vec and the checkpoint inhibitor ipilimumab (anti-CTLA-4), researchers have postulated that their combined effect on the immune microenvironment and T cell modulation may be greater than either of the monotherapies. In a phase Ib study, T-Vec was combined with ipilimumab in patients with untreated stage IIIB or IV melanoma. The objective response rate was 50% and 44% of patients enrolled had a durable response that lasted at least 6 months . There were no dose-limiting toxicities and overall adverse event rates were comparable to that of ipilimumab monotherapy. Interestingly, the authors observed that patients who demonstrated better disease control had increased levels of activated CD8+ T cells (flow cytometry analysis of whole blood samples) when compared to those patients who had disease progression following T-Vec monotherapy . This difference did decrease following ipilimumab, and consequently the authors suggest that T-Vec might stimulate a different subset of T cells that could generate a more specific anti-tumor response when compared to those stimulated by ipilimumab . In the subsequent phase II trial, 39% of patients in the combination arm (T-Vec + ipilimumab) demonstrated an objective response while only 18% of patients in the ipilimumab arm demonstrated a response (p = 0.002) . Additionally, distant un-injected sites demonstrated abscopal responses as decreases in visceral lesions were demonstrated in 52% of patients in the combination arm but only in 23% of patients in the ipilimumab arm . The combination of T-Vec with an anti-CTLA-4 antibody has demonstrated a greater efficacy while still maintaining tolerability and has the potential to become a standard therapy for patients with advanced melanoma.
T-Vec is also being tested in combination with an anti-PD1 antibody (pembrolizumab) for the treatment of melanoma patients. The ongoing Masterkey-265 trial was designed as a phase Ib/III trial, and thus far results have been promising (NCT 02263508). In the phase Ib study, there were no dose limiting toxicities and the objective response rate was 62% with a complete response rate of 33% . The authors demonstrated increased levels of PD-L1 expression, increased amounts of circulating CD8+ and CD4+ T cells, and increased inflammation at tumor sites distant from the injected lesions (even in patients with low levels of tumor infiltrating lymphocytes [TIL]) . These analyses were performed prior to anti-PD1 antibody delivery, which suggests that the oncolytic virus can change the immune cell makeup surrounding the tumor which is then more conducive to combination therapy with a checkpoint inhibitor. A phase III study is ongoing and the results are highly anticipated.
HF 10 is another oncolytic virus in the HSV family. It differs from T-Vec in that it is a spontaneously mutated virus without any insertions of foreign genes. Natural deletions and insertions resulted in an overexpression of UL53 and UL54 as well as a loss of expression of UL43, UL49.5, UL55, and UL 56 . This has translated into high innate tumor tropism, a high degree of viral replication, and potent antitumor efficacy across a variety of malignancies . It has been used in a phase II clinical trial with patients who have Stage IIIB/C or IV unresectable melanoma in combination with ipilimumab (NCT 02272855). There were no disease limiting toxicities, and the best overall response rate was 41%, while the disease stability rate was 68% .
The Adenoviridae family consists of non-enveloped viruses with double-stranded DNA genomes that are surrounded by an icosahedral capsid . These viruses range from 70 to 90 nm in size and possess a genome of approximately 35 kb that is relatively easy to modify and lends itself well to the insertion of transgenes . From the 57 known serotypes of adenovirus (divided into categories A–G based upon their agglutination properties and oncogenic potential in rodent models), serotype 5 from group C has been one of the most commonly used backbones in oncolytic viruses .
This is a replication competent oncolytic adenovirus with enhancements to confer increased infectivity as well as tumor selectivity . The selective replication of the vector results from a 24 base pair deletion in the E1A region of the adenoviral genome, which allows the virus to replicate in cancer cells that lack a functional Rb pathway, but not in normal cells . The vector was tested in a phase I trial for thirty-seven patients with recurrent malignant glioma. One group (n = 25, group A) underwent intratumoral injections to evaluate dosing and response across different viral titers, while the other group (n = 12, group B) underwent intratumoral injection via implanted catheter and subsequent surgical resection. Tumor size reductions were documented in 72% of patients in Group A with a median overall survival time of 9.5 months . Immunohistochemical analysis of resected specimens demonstrated decreases in the expression of TIM-3, but none of the other checkpoint proteins including PD-1 or PD-L1 . T-cell exhaustion is one of the ways that tumor cells can create a locally immunosuppressed environment, and it is known that inhibitory receptors (e.g. TIM-3, PD-1) can provide some regulation of these exhausted T cells . The authors suggest that since viral inoculation with DNX-2401 may partially overcome some aspects of T-cell exhaustion and have subsequently used this as a rationale to investigate the virus in combination with anti-PD-1 antibodies . The CAPTIVE trial is an ongoing phase II study employing the virus and pembrolizumab in patients who have had glioblastoma that has progressed after initial therapy (NCT 02798406).
ONCOS-102 (Ad 5/3 Δ24 GM CSF)
ONCOS-102 is a serotype 5 adenovirus with multiple modifications including a chimeric 5/3 fiber-knob region to augment infectivity, a 24 base pair deletion in the E1a region conferring selective replication in Rb-pathway deficient cells, and expression of GM-CSF to boost the immune cell infiltrate at the site of viral inoculation . This virus has been extensively tested in a pre-clinical setting and has already progressed to phase I trials. In a study of 12 patients with treatment refractory solid tumors (including mesothelioma, sarcoma, ovarian, colorectal, liver and lung cancers), there were no grade 4/5 adverse events following intratumoral injections . Inoculation with the vector resulted in a profound immune cell infiltration to the tumor. When compared to pre-treatment biopsies, ONCOS-102 resulted in a 5.9 times increase in the expression of CD3 (a T cell marker) and a four-fold increase in CD8+ cells in the tumor on post-treatment biopsies . Additionally, two patients (one with mesothelioma, the other with ovarian cancer) developed systemic anti-tumor immunity as demonstrated through the comparison of pre and post treatment peripheral blood mononuclear cells (PBMC) in blood samples to determine the specificity of CD8+ T-cells for cancer-testis (CT) antigens . Here, the mesothelioma patient demonstrated a profound induction of MAGE-A3-specific CD8+ T cells, and the ovarian cancer patient demonstrated CD8+ T cells that were specific for NY-ESO-1 [62, 63]. Furthermore, two of the patients with mesothelioma demonstrated increased levels of PD-L1 expression in the tumors following treatment with ONCOS-102 . These observations serve as the basis for potential combination therapies with checkpoint inhibitors, and there is an ongoing clinical trial utilizing ONCOS-102 with pembrolizumab in patients with advanced melanoma who have progressed after PD1 blockade (NCT 03003676).
Thus far, we have only discussed approaches whereby the drug of interest (checkpoint inhibitor) is given separately from the oncolytic vector. This has been described as a ‘trans approach’ while a ‘cis approach’ would consist of a gene encoding the product of interest (human monoclonal antibody for anti-PD1 or anti-CTLA-4) being inserted into the viral genome .
The Hemminki group has reported an oncolytic adenovirus that included a transgene expressing an antibody specific for CTLA-4 . The vector (Ad 5/3 Δ24a CTLA4) was shown to produce a high level of the human monoclonal antibody against CTLA4, which was effective at inducing T cell activity . Du et al. also reported construction of an oncolytic adenovirus with an anti-CTLA4 antibody inserted into the E3 region (SKL002), which was efficacious in both in vitro and in vivo models . This vector was under the control of the E2F-1 promoter, which resulted in selective replication in cancer cells deficient in the Rb pathway. Notably, the vector demonstrated strong in vivo effects in subcutaneous mouse models for lung cancer and melanoma.
Vaccinia viruses are members of the Poxviridae family and have large (~ 190 kb) double-stranded DNA genomes that are suitable for transgene insertion . These viruses replicate in the host cytoplasm and so the risk of insertional mutagenesis is all but eliminated . Due to its role in smallpox vaccination programs, the potential for immune system stimulation and the safety profile of this vector system is well documented . These viruses do not have a specific cell-surface receptor required for entry into the host, which contributes to the natural tropism for a variety of cancer cells and makes it an attractive backbone for oncolytic virotherapy .
Pexa-Vec (pexastimogene devacirepvec, JX-594) is an oncolytic vaccinia virus that expresses the human GM-CSF and beta-galactosidase transgenes . In addition, it has an inactivated thymidine kinase gene that provides for selective replication that is dependent upon high levels of thymine production, which is common to many cancer cells . In addition to its oncolytic and immunostimulatory properties, Pexa-Vec is also known to target vascular cells within the tumor. It has been demonstrated in laboratory and in human studies that this virus is capable of targeting and infecting tumor associated endothelial cells, which ultimately results in vascular disruption and oncolysis . The vector has been tested in multiple clinical trials and has been shown to be well tolerated with antitumor activity across a range of solid malignancies [73,74,75,76]. In a recent abstract presented at the 2018 ASCO meeting, Anthoney et al. presented data in patients with metastatic liver tumors who were given a single dose of intravenous (IV) Pexa-Vec and then underwent surgical resection . They demonstrated a robust activation of natural killer cells, antigen presenting cells, and CD4/CD8+ T cells. The study will go on to explore combinations of the viral vector with nivolumab for the treatment of liver tumors (NCT 03071094). Furthermore, Pexa-Vec is currently being studied in conjunction with immune checkpoint inhibitors in two additional ongoing clinical trials for colorectal cancer and other advanced solid tumors (NCT 03206073, NCT 02977156).
Reoviruses are members of the Reoviridae family and are typically 75–85 nm in diameter . They are non-enveloped viruses with icosahedral capsids and double-stranded RNA genomes . Reoviruses replicate in the cytoplasm and produce viral RNAs that activate the PKR (protein kinase R) pathway . Interestingly, in Ras-transformed cells, the PKR pathway is inhibited which results in the release of translational inhibition and serves to augment the replication and oncolysis of reoviruses . Given the number of cancers with Ras mutations, the natural tropism of reovirus for these tumors makes it a versatile backbone for oncolytic vector design .
Recent preclinical data supports the use of the combination of reovirus and anti-PD-1 antibodies. In subcutaneous melanoma tumors established in immunocompetent mice, the combination of intratumoral reovirus and intravenous anti-PD-1 antibody (delivered 7 days after first viral dose) resulted in statistically significant prolonged survival compared to either monotherapy treatment . The authors also showed that PD-1 blockade enhanced the CD8+ T cell Th1 antitumor response (primed by the reovirus injection) and also augmented NK-cell recognition of reovirus-infected cells . Similar findings were obtained with intravenous reovirus injection in patients with intracranial glioblastomas. In a phase Ib trial, patients who were undergoing debulking neurosurgery underwent a reovirus infusion prior to resection. When compared to control cases, these patients demonstrated a marked increase in tumor-infiltrating cytotoxic T cells (CD8+) on immunohistochemical staining of the resected specimens . A clinical trial (NCT 02620423) investigating the use of pelareorep in combination with pembrolizumab and chemotherapy in patients with relapsed metastatic pancreatic adenocarcinoma is ongoing. A recent abstract reported the preliminary data for 11 patients treated with pembrolizumab, pelareorep, and gemcitabine. The authors note manageable toxicities and 3 out of 5 patients who were eligible for efficacy evaluation demonstrated a partial response or stable disease .
These viruses are members of the Picornaviridae family and are non-enveloped with a single-stranded RNA genome . They are small viruses that are approximately 30 nm in size and have an icosahedral capsid structure . There are two subgroups of Coxsackieviruses which are categorized based on their effects in murine models and consist of twenty-three unique serotypes in Group A and six serotypes in Group B [16, 85].
CVA 21 (Cavatak)
This coxsackie vector is the twenty-first serotype from Group A and has not been modified with any deletions or transgene insertions . Its binding to cancer cells is mediated through intercellular adhesion molecule 1 (ICAM-1) and decay-accelerating factor (DAF) . Since melanoma cells are known to overexpress ICAM-1, metastatic melanoma was one of the first targets for this oncolytic vector [87, 88]. Additionally, other serotypes of coxsackieviruses including CVA 13, CVA15, and CVA 18 are currently being explored for their potential as oncolytic vectors .
Additional pre-clinical studies have demonstrated an immune component to the anti-tumor response represented by increases in IL-8 and gamma-interferon in melanoma patients . In addition, the vector was quite effective when combined with anti-PD-1 or anti-CTLA-4 antibodies . Results of an extension study to the CALM clinical trial (NCT 01636882) showed increased immune cell infiltrates and expression of checkpoint molecules in patients receiving intratumoral injections of the CVA 21 virus . These observations and the strong cytocidal effects have led to multiple clinical trials, many of which have combined CVA 21 with immune checkpoint inhibitors [93,94,95]. For example, in the CAPRA clinical trial (single arm, multi-institutional, phase Ib, NCT 02565992) where patients received multiple intratumoral injections of CVA21 as well as multiple doses of pembrolizumab, there was an objective response rate of 73% .
Summary and conclusions
The last decade has ushered in a new age of cancer care due to the mainstream adoption of immunotherapies. Checkpoint inhibitors have revolutionized the treatment of patients with melanoma and other malignancies. Similarly, with the FDA approval of T-Vec, oncolytic virotherapy gained a major victory. As researchers have learned more about the mechanism of action of viral oncolysis, it has become clear that the immune component is equally important (if not more so) than direct lysis. Consequently, the combinations of viral vectors with agents that influence the tumor immune microenvironment and help to augment T cell responses have incredible potential. Multiple vector systems are currently being tested in clinical trials in combination with anti-PD-1 or anti-CTLA-4 antibodies, and thus far the results have been encouraging. Additionally, many research groups are exploring similar treatment schemes with other vectors systems in preclinical settings, some of which include measles virus [96, 97], vesicular stomatitis virus (VSV) [98, 99], newcastle disease virus (NDV) [100, 101], semlicki forest virus (SFV) , and parvovirus . With time, it is expected that additional oncolytic vectors with be tested with checkpoint inhibitors in clinical trials.
As these types of combination therapies progress in development, important points will need to be addressed. Will it be more beneficial to have the vector express a transgene which encodes for an anti-PD-1/CTLA-4 antibody or will concurrent IV delivery of such an antibody be best? If the latter is to be pursued, what will be the optimal timing of the antibody delivery?
Oncolytic vectors have the ability to lyse target cells as part of the replication cycle, but they also have powerful immunomodulatory effects. They have been shown to induce both innate and adaptive tumor responses as well as prime cancer cells for treatments with additional agents. To this end, multiple mechanisms (innate, adaptive, and acquired) of resistance to immunotherapy have been identified and oncolytic vectors are suited to be part of the solution to these hurdles in treatment [39, 104]. Multiple groups have demonstrated that local oncolytic virus injection can modulate tumor-specific CD8+ T-cell responses to make distant tumors increasingly susceptible to immune checkpoint inhibitor therapy [100, 105]. Results of ongoing clinical trials in patients who have progressed after immune checkpoint inhibition (e.g. NCT 03003676) will shed additional light on oncolytic virotherapy’s role in helping to overcome resistance to immunotherapy. By harnessing the potential of the combination of viral vectors and checkpoint inhibitors, great strides can be made in further developing treatment regimens employing these novel therapeutics to improve patient outcomes.
triple negative breast cancer
renal cell cancer
non-small cell lung cancer
head and neck squamous cell carcinoma
Hodgkin lymphoma (classic)
primary mediastinal B cell lymphoma
microsatellite instability high
mismatch repair gene deficient
cytotoxic T lymphocyte-associated antigen-4
programmed death receptor 1
programmed death receptor ligand 1
granulocyte macrophage colony stimulating factor
immunogenic cell death
damage associated molecular pattern
pathogen-associated molecular pattern
pattern recognition receptor
antigen presenting cell
peripheral blood mononuclear cell
myeloid derived suppressor cell
intercellular adhesion molecule
decay accelerating factor
human telomerase reverse transcriptase
sodium iodide symporter
plaque forming unit
tissue culture infective dose
stereotactic body radiotherapy
herpes simplex virus
vesicular stomatitis virus
newcastle disease virus
semlicki forest virus
Burstein HJ, Krilov L, Aragon-Ching JB, Baxter NN, Chiorean EG, Chow WA, De Groot JF, Devine SM, DuBois SG, El-Deiry WS et al (2017) Clinical cancer advances 2017: annual report on progress against cancer from the american society of clinical oncology. J Clin Oncol 35(12):1341–1367
Dizon DS, Krilov L, Cohen E, Gangadhar T, Ganz PA, Hensing TA, Hunger S, Krishnamurthi SS, Lassman AB, Markham MJ et al (2016) Clinical cancer advances 2016: annual report on progress against cancer from the american society of clinical oncology. J Clin Oncol 34(9):987–1011
Heymach J, Krilov L, Alberg A, Baxter N, Chang SM, Corcoran R, Dale W, DeMichele A, Magid Diefenbach CS, Dreicer R et al (2018) Clinical cancer advances 2018: annual report on progress against cancer from the american society of clinical oncology. J Clin Oncol 36(10):1020–1044
Kyi C, Postow MA (2016) Immune checkpoint inhibitor combinations in solid tumors: opportunities and challenges. Immunotherapy 8(7):821–837
Camacho LH (2015) CTLA-4 blockade with ipilimumab: biology, safety, efficacy, and future considerations. Cancer Med 4(5):661–672
Khoja L, Butler MO, Kang SP, Ebbinghaus S, Joshua AM (2015) Pembrolizumab. J Immunother Cancer 3:36
Hazama S, Tamada K, Yamaguchi Y, Kawakami Y, Nagano H (2018) Current status of immunotherapy against gastrointestinal cancers and its biomarkers: perspective for precision immunotherapy. Ann Gastroenterol Surg 2(4):289–303
Larkin J, Chiarion-Sileni V, Gonzalez R, Grob JJ, Cowey CL, Lao CD, Schadendorf D, Dummer R, Smylie M, Rutkowski P et al (2015) Combined nivolumab and ipilimumab or monotherapy in untreated melanoma. N Engl J Med 373(1):23–34
Wolchok JD, Chiarion-Sileni V, Gonzalez R, Rutkowski P, Grob JJ, Cowey CL, Lao CD, Wagstaff J, Schadendorf D, Ferrucci PF et al (2017) Overall survival with combined nivolumab and ipilimumab in advanced melanoma. N Engl J Med 377(14):1345–1356
Warner SG, O’Leary MP, Fong Y (2017) Therapeutic oncolytic viruses: clinical advances and future directions. Curr Opin Oncol 29(5):359–365
Russell SJ, Peng KW, Bell JC (2012) Oncolytic virotherapy. Nat Biotechnol 30(7):658–670
Lichty BD, Breitbach CJ, Stojdl DF, Bell JC (2014) Going viral with cancer immunotherapy. Nat Rev Cancer 14(8):559–567
Liu Z, Ravindranathan R, Kalinski P, Guo ZS, Bartlett DL (2017) Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy. Nat Commun 8:14754
Chen L, Han X (2015) Anti-PD-1/PD-L1 therapy of human cancer: past, present, and future. J Clin Invest 125(9):3384–3391
Hardcastle J, Kurozumi K, Chiocca EA, Kaur B (2007) Oncolytic viruses driven by tumor-specific promoters. Curr Cancer Drug Targets 7(2):181–189
Kaufman HL, Kohlhapp FJ, Zloza A (2015) Oncolytic viruses: a new class of immunotherapy drugs. Nat Rev Drug Discov 14(9):642–662
LaRocca CJ, Han J, Gavrikova T, Armstrong L, Oliveira AR, Shanley R, Vickers SM, Yamamoto M, Davydova J (2015) Oncolytic adenovirus expressing interferon alpha in a syngeneic Syrian hamster model for the treatment of pancreatic cancer. Surgery 157(5):888–898
Latham JP, Searle PF, Mautner V, James ND (2000) Prostate-specific antigen promoter/enhancer driven gene therapy for prostate cancer: construction and testing of a tissue-specific adenovirus vector. Cancer Res 60(2):334–341
Doloff JC, Waxman DJ, Jounaidi Y (2008) Human telomerase reverse transcriptase promoter-driven oncolytic adenovirus with E1B-19 kDa and E1B-55 kDa gene deletions. Hum Gene Ther 19(12):1383–1400
Ottolino-Perry K, Diallo JS, Lichty BD, Bell JC, McCart JA (2010) Intelligent design: combination therapy with oncolytic viruses. Mol Ther 18(2):251–263
Steinwaerder DS, Carlson CA, Lieber A (2001) Human papilloma virus E6 and E7 proteins support DNA replication of adenoviruses deleted for the E1A and E1B genes. Mol Ther 4(3):211–216
LaRocca CJ, Han J, Salzwedel AO, Davydova J, Herzberg MC, Gopalakrishnan R, Yamamoto M (2016) Oncolytic adenoviruses targeted to Human Papilloma Virus-positive head and neck squamous cell carcinomas. Oral Oncol 56:25–31
Huang JL, LaRocca CJ, Yamamoto M (2016) Showing the way: oncolytic adenoviruses as chaperones of immunostimulatory adjuncts. Biomedicines 4(3):23
Armstrong L, Arrington A, Han J, Gavrikova T, Brown E, Yamamoto M, Vickers SM, Davydova J (2012) Generation of a novel, cyclooxygenase-2-targeted, interferon-expressing, conditionally replicative adenovirus for pancreatic cancer therapy. Am J Surg 204(5):741–750
Toda M, Martuza RL, Rabkin SD (2000) Tumor growth inhibition by intratumoral inoculation of defective herpes simplex virus vectors expressing granulocyte-macrophage colony-stimulating factor. Mol Ther 2(4):324–329
Haddad D, Fong Y (2015) Molecular imaging of oncolytic viral therapy. Mol Ther Oncolytics 1:14007
Li H, Peng KW, Russell SJ (2012) Oncolytic measles virus encoding thyroidal sodium iodide symporter for squamous cell cancer of the head and neck radiovirotherapy. Hum Gene Ther 23(3):295–301
Maroun J, Munoz-Alia M, Ammayappan A, Schulze A, Peng KW, Russell S (2017) Designing and building oncolytic viruses. Future Virol 12(4):193–213
Raki M, Sarkioja M, Escutenaire S, Kangasniemi L, Haavisto E, Kanerva A, Cerullo V, Joensuu T, Oksanen M, Pesonen S et al (2011) Switching the fiber knob of oncolytic adenoviruses to avoid neutralizing antibodies in human cancer patients. J Gene Med 13(5):253–261
Garcia-Castro J, Alemany R, Cascallo M, Martinez-Quintanilla J, Arriero Mdel M, Lassaletta A, Madero L, Ramirez M (2010) Treatment of metastatic neuroblastoma with systemic oncolytic virotherapy delivered by autologous mesenchymal stem cells: an exploratory study. Cancer Gene Ther 17(7):476–483
Guo ZS, Parimi V, O’Malley ME, Thirunavukarasu P, Sathaiah M, Austin F, Bartlett DL (2010) The combination of immunosuppression and carrier cells significantly enhances the efficacy of oncolytic poxvirus in the pre-immunized host. Gene Ther 17(12):1465–1475
Green DR, Ferguson T, Zitvogel L, Kroemer G (2009) Immunogenic and tolerogenic cell death. Nat Rev Immunol 9(5):353–363
Guo ZS, Liu Z, Bartlett DL (2014) Oncolytic immunotherapy: dying the right way is a key to eliciting potent antitumor immunity. Front Oncol 4:74
Medzhitov R, Janeway CA Jr (2002) Decoding the patterns of self and nonself by the innate immune system. Science 296(5566):298–300
Melero I, Hervas-Stubbs S, Glennie M, Pardoll DM, Chen L (2007) Immunostimulatory monoclonal antibodies for cancer therapy. Nat Rev Cancer 7(2):95–106
Brunet JF, Denizot F, Luciani MF, Roux-Dosseto M, Suzan M, Mattei MG, Golstein P (1987) A new member of the immunoglobulin superfamily—CTLA-4. Nature 328(6127):267–270
Ishida Y, Agata Y, Shibahara K, Honjo T (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J 11(11):3887–3895
Berkey SE, Thorne SH, Bartlett DL (2017) Oncolytic virotherapy and the tumor microenvironment. Adv Exp Med Biol 1036:157–172
Sharma P, Hu-Lieskovan S, Wargo JA, Ribas A (2017) Primary, adaptive, and acquired resistance to cancer immunotherapy. Cell 168(4):707–723
Gujar S, Pol JG, Kim Y, Lee PW, Kroemer G (2018) Antitumor benefits of antiviral immunity: an underappreciated aspect of oncolytic virotherapies. Trends Immunol 39(3):209–221
Ribas A, Dummer R, Puzanov I, VanderWalde A, Andtbacka RHI, Michielin O, Olszanski AJ, Malvehy J, Cebon J, Fernandez E et al (2017) Oncolytic virotherapy promotes intratumoral t cell infiltration and improves anti-PD-1 immunotherapy. Cell 170(6):1109–1119
Samson A, Scott KJ, Taggart D, West EJ, Wilson E, Nuovo GJ, Thomson S, Corns R, Mathew RK, Fuller MJ et al (2018) Intravenous delivery of oncolytic reovirus to brain tumor patients immunologically primes for subsequent checkpoint blockade. Sci Transl Med 10(422):7577
Grinde B (2013) Herpesviruses: latency and reactivation—viral strategies and host response. J Oral Microbiol 5:22766
Sanchala DS, Bhatt LK, Prabhavalkar KS (2017) Oncolytic herpes simplex viral therapy: a stride toward selective targeting of cancer cells. Front Pharmacol 8:270
Liu BL, Robinson M, Han ZQ, Branston RH, English C, Reay P, McGrath Y, Thomas SK, Thornton M, Bullock P et al (2003) ICP345 deleted herpes simplex virus with enhanced oncolytic, immune stimulating, and anti-tumour properties. Gene Ther 10(4):292–303
Kohlhapp FJ, Kaufman HL (2016) Molecular pathways: mechanism of action for talimogene laherparepvec, a new oncolytic virus immunotherapy. Clin Cancer Res 22(5):1048–1054
Hu JC, Coffin RS, Davis CJ, Graham NJ, Groves N, Guest PJ, Harrington KJ, James ND, Love CA, McNeish I et al (2006) A phase I study of OncoVEXGM-CSF, a second-generation oncolytic herpes simplex virus expressing granulocyte macrophage colony-stimulating factor. Clin Cancer Res 12(22):6737–6747
Andtbacka RH, Kaufman HL, Collichio F, Amatruda T, Senzer N, Chesney J, Delman KA, Spitler LE, Puzanov I, Agarwala SS et al (2015) Talimogene laherparepvec improves durable response rate in patients with advanced melanoma. J Clin Oncol 33(25):2780–2788
Kaufman HL, Kim DW, DeRaffele G, Mitcham J, Coffin RS, Kim-Schulze S (2010) Local and distant immunity induced by intralesional vaccination with an oncolytic herpes virus encoding GM-CSF in patients with stage IIIc and IV melanoma. Ann Surg Oncol 17(3):718–730
Puzanov I, Milhem MM, Minor D, Hamid O, Li A, Chen L, Chastain M, Gorski KS, Anderson A, Chou J et al (2016) Talimogene laherparepvec in combination with ipilimumab in previously untreated, unresectable stage IIIB–IV melanoma. J Clin Oncol 34(22):2619–2626
Chesney J, Puzanov I, Collichio F, Singh P, Milhem MM, Glaspy J, Hamid O, Ross M, Friedlander P, Garbe C et al (2018) Randomized, open-label phase II study evaluating the efficacy and safety of talimogene laherparepvec in combination with ipilimumab versus ipilimumab alone in patients with advanced, unresectable melanoma. J Clin Oncol 36(17):1658–1667
Eissa IR, Naoe Y, Bustos-Villalobos I, Ichinose T, Tanaka M, Zhiwen W, Mukoyama N, Morimoto T, Miyajima N, Hitoki H et al (2017) Genomic signature of the natural oncolytic herpes simplex virus HF10 and its therapeutic role in preclinical and clinical trials. Front Oncol 7:149
Andtbacka RHI, Ross MI, Agarwala SS, Taylor MH, Vetto JT, Neves RI, Daud A, Khong HT, Ungerleider RS, Tanaka M et al (2017) Final results of a phase II multicenter trial of HF10, a replication-competent HSV-1 oncolytic virus, and ipilimumab combination treatment in patients with stage IIIB–IV unresectable or metastatic melanoma. J Clin Oncol 35(15_suppl):9510
Khanal S, Ghimire P, Dhamoon AS (2018) The repertoire of adenovirus in human disease: the innocuous to the deadly. Biomedicines 6(1):30
Buijs PR, Verhagen JH, van Eijck CH, van den Hoogen BG (2015) Oncolytic viruses: from bench to bedside with a focus on safety. Hum Vaccin Immunother 11(7):1573–1584
Fueyo J, Alemany R, Gomez-Manzano C, Fuller GN, Khan A, Conrad CA, Liu TJ, Jiang H, Lemoine MG, Suzuki K et al (2003) Preclinical characterization of the antiglioma activity of a tropism-enhanced adenovirus targeted to the retinoblastoma pathway. J Natl Cancer Inst 95(9):652–660
Fueyo J, Gomez-Manzano C, Alemany R, Lee PS, McDonnell TJ, Mitlianga P, Shi YX, Levin VA, Yung WK, Kyritsis AP (2000) A mutant oncolytic adenovirus targeting the Rb pathway produces anti-glioma effect in vivo. Oncogene 19(1):2–12
Lang FF, Conrad C, Gomez-Manzano C, Yung WKA, Sawaya R, Weinberg JS, Prabhu SS, Rao G, Fuller GN, Aldape KD et al (2018) Phase I study of DNX-2401 (Delta-24-RGD) oncolytic adenovirus: replication and immunotherapeutic effects in recurrent malignant glioma. J Clin Oncol 36(14):1419–1427
Wherry EJ, Kurachi M (2015) Molecular and cellular insights into T cell exhaustion. Nat Rev Immunol 15(8):486–499
Koski A, Kangasniemi L, Escutenaire S, Pesonen S, Cerullo V, Diaconu I, Nokisalmi P, Raki M, Rajecki M, Guse K et al (2010) Treatment of cancer patients with a serotype 5/3 chimeric oncolytic adenovirus expressing GMCSF. Mol Ther 18(10):1874–1884
Ranki T, Pesonen S, Hemminki A, Partanen K, Kairemo K, Alanko T, Lundin J, Linder N, Turkki R, Ristimaki A et al (2016) Phase I study with ONCOS-102 for the treatment of solid tumors—an evaluation of clinical response and exploratory analyses of immune markers. J Immunother Cancer 4:17
Ranki T, Joensuu T, Jager E, Karbach J, Wahle C, Kairemo K, Alanko T, Partanen K, Turkki R, Linder N et al (2014) Local treatment of a pleural mesothelioma tumor with ONCOS-102 induces a systemic antitumor CD8(+) T-cell response, prominent infiltration of CD8(+) lymphocytes and Th1 type polarization. Oncoimmunology 3(10):e958937
Vassilev L, Ranki T, Joensuu T, Jager E, Karbach J, Wahle C, Partanen K, Kairemo K, Alanko T, Turkki R et al (2015) Repeated intratumoral administration of ONCOS-102 leads to systemic antitumor CD8(+) T-cell response and robust cellular and transcriptional immune activation at tumor site in a patient with ovarian cancer. Oncoimmunology 4(7):e1017702
Martin NT, Bell JC (2018) Oncolytic virus combination therapy: killing one bird with two stones. Mol Ther 26(6):1414–1422
Dias JD, Hemminki O, Diaconu I, Hirvinen M, Bonetti A, Guse K, Escutenaire S, Kanerva A, Pesonen S, Loskog A et al (2012) Targeted cancer immunotherapy with oncolytic adenovirus coding for a fully human monoclonal antibody specific for CTLA-4. Gene Ther 19(10):988–998
Du T, Shi G, Li YM, Zhang JF, Tian HW, Wei YQ, Deng H, Yu DC (2014) Tumor-specific oncolytic adenoviruses expressing granulocyte macrophage colony-stimulating factor or anti-CTLA4 antibody for the treatment of cancers. Cancer Gene Ther 21(8):340–348
Haddad D (2017) Genetically engineered vaccinia viruses as agents for cancer treatment, imaging, and transgene delivery. Front Oncol 7:96
Thorne SH (2014) Immunotherapeutic potential of oncolytic vaccinia virus. Front Oncol 4:155
Al Yaghchi C, Zhang Z, Alusi G, Lemoine NR, Wang Y (2015) Vaccinia virus, a promising new therapeutic agent for pancreatic cancer. Immunotherapy 7(12):1249–1258
Breitbach CJ, Bell JC, Hwang TH, Kirn DH, Burke J (2015) The emerging therapeutic potential of the oncolytic immunotherapeutic Pexa-Vec (JX-594). Oncolytic Virother 4:25–31
Hengstschlager M, Pfeilstocker M, Wawra E (1998) Thymidine kinase expression. A marker for malignant cells. Adv Exp Med Biol 431:455–460
Breitbach CJ, Arulanandam R, De Silva N, Thorne SH, Patt R, Daneshmand M, Moon A, Ilkow C, Burke J, Hwang TH et al (2013) Oncolytic vaccinia virus disrupts tumor-associated vasculature in humans. Cancer Res 73(4):1265–1275
Mastrangelo MJ, Maguire HC Jr, Eisenlohr LC, Laughlin CE, Monken CE, McCue PA, Kovatich AJ, Lattime EC (1999) Intratumoral recombinant GM-CSF-encoding virus as gene therapy in patients with cutaneous melanoma. Cancer Gene Ther 6(5):409–422
Breitbach CJ, Burke J, Jonker D, Stephenson J, Haas AR, Chow LQ, Nieva J, Hwang TH, Moon A, Patt R et al (2011) Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans. Nature 477(7362):99–102
Hwang TH, Moon A, Burke J, Ribas A, Stephenson J, Breitbach CJ, Daneshmand M, De Silva N, Parato K, Diallo JS et al (2011) A mechanistic proof-of-concept clinical trial with JX-594, a targeted multi-mechanistic oncolytic poxvirus, in patients with metastatic melanoma. Mol Ther 19(10):1913–1922
Park BH, Hwang T, Liu TC, Sze DY, Kim JS, Kwon HC, Oh SY, Han SY, Yoon JH, Hong SH et al (2008) Use of a targeted oncolytic poxvirus, JX-594, in patients with refractory primary or metastatic liver cancer: a phase I trial. Lancet Oncol 9(6):533–542
Anthoney A, Samson A, West E, Turnbull SJ, Scott K, Tidswell E, Kingston J, Johnpulle M, Noutch S, Bendjama K et al (2018) Single intravenous preoperative administration of the oncolytic virus Pexa-Vec to prime anti-tumor immunity. J Clin Oncol 36(15_Suppl):3092
Phillips MB, Stuart JD, Rodriguez Stewart RM, Berry JT, Mainou BA, Boehme KW (2018) Current understanding of reovirus oncolysis mechanisms. Oncolytic Virother 7:53–63
Strong JE, Coffey MC, Tang D, Sabinin P, Lee PW (1998) The molecular basis of viral oncolysis: usurpation of the Ras signaling pathway by reovirus. EMBO J 17(12):3351–3362
Gong J, Sachdev E, Mita AC, Mita MM (2016) Clinical development of reovirus for cancer therapy: an oncolytic virus with immune-mediated antitumor activity. World J Methodol 6(1):25–42
Chakrabarty R, Tran H, Selvaggi G, Hagerman A, Thompson B, Coffey M (2015) The oncolytic virus, pelareorep, as a novel anticancer agent: a review. Invest New Drugs 33(3):761–774
Rajani K, Parrish C, Kottke T, Thompson J, Zaidi S, Ilett L, Shim KG, Diaz RM, Pandha H, Harrington K et al (2016) Combination therapy With reovirus and anti-PD-1 blockade controls tumor growth through innate and adaptive immune responses. Mol Ther 24(1):166–174
Mahalingam D, Fountzilas C, Moseley JL, Noronha N, Cheetham K, Dzugalo A, Nuovo G, Gutierrez A, Arora SP (2018) A study of pelareorep in combination with pembrolizumab and chemotherapy in patients (pts) with relapsed metastatic adenocarcinoma of the pancreas (MAP). J Clin Oncol 36(4_Suppl):283
Lundstrom K (2018) New frontiers in oncolytic viruses: optimizing and selecting for virus strains with improved efficacy. Biologics 12:43–60
Bradley S, Jakes AD, Harrington K, Pandha H, Melcher A, Errington-Mais F (2014) Applications of coxsackievirus A21 in oncology. Oncolytic Virother 3:47–55
Shafren DR, Dorahy DJ, Ingham RA, Burns GF, Barry RD (1997) Coxsackievirus A21 binds to decay-accelerating factor but requires intercellular adhesion molecule 1 for cell entry. J Virol 71(6):4736–4743
Shafren DR, Au GG, Nguyen T, Newcombe NG, Haley ES, Beagley L, Johansson ES, Hersey P, Barry RD (2004) Systemic therapy of malignant human melanoma tumors by a common cold-producing enterovirus, coxsackievirus a21. Clin Cancer Res 10(1 Pt 1):53–60
Au GG, Lindberg AM, Barry RD, Shafren DR (2005) Oncolysis of vascular malignant human melanoma tumors by Coxsackievirus A21. Int J Oncol 26(6):1471–1476
Au GG, Beagley LG, Haley ES, Barry RD, Shafren DR (2011) Oncolysis of malignant human melanoma tumors by Coxsackieviruses A13, A15 and A18. Virol J 8:22
Andtbacka RH, Shafren DR, Grose M, Post L, Weisberg J (2014) Abstract 2939: cAVATAK-mediated oncolytic immunotherapy in advanced melanoma patients. Can Res 74(19 Supplement):2939
Yuan Quah M, Wong Y, Andtbacka R, Au G, Shafren DR (2016) Abstract 2341: elevated immune activity following an anticancer combination therapy of a novel oncolytic immunotherapeutic agent, CAVATAK (Coxsackievirus A21), and immune checkpoint blockade. Can Res 76(14 Supplement):2341
Andtbacka R, Curti B, Hallmeyer S, Peng Z, Paustian C, Bifulco C, Fox B, Grose M, Shafren DR (2016) Abstract CT053: Intratumoral coxsackievirus A21 increases immune-cell infiltrates and upregulates immune-checkpoint molecules in the tumor microenvironment of advanced melanoma patients: phase II CALM extension study. Cancer Res 76(14 Supplement):CT053
Pandha H, Harrington K, Ralph C, Melcher A, Gupta S, Akerley W, Sandborn RE, Rudin C, Rosenberg J, Kaufman D et al (2017) Abstract CT115: phase 1b KEYNOTE 200 (STORM study): A study of an intravenously delivered oncolytic virus, Coxsackievirus A21 in combination with pembrolizumab in advanced cancer patients. Cancer Res 77(13 Supplement):CT115
Curti B, Richards J, Hallmeyer S, Faries M, Andtbacka R, Daniels G, Grose M, Shafren DR (2017) Abstract CT114: the MITCI (Phase 1b) study: a novel immunotherapy combination of intralesional Coxsackievirus A21 and systemic ipilimumab in advanced melanoma patients with or without previous immune checkpoint therapy treatment. Cancer Res 77(13 Supplement):CT114
Silk AW, Kaufman H, Gabrail N, Mehnert J, Bryan J, Norrell J, Medina D, Bommareddy P, Shafren D, Grose M et al (2017) Abstract CT026: phase 1b study of intratumoral Coxsackievirus A21 (CVA21) and systemic pembrolizumab in advanced melanoma patients: interim results of the CAPRA clinical trial. Cancer Res 77(13 Supplement):CT026
Engeland CE, Grossardt C, Veinalde R, Bossow S, Lutz D, Kaufmann JK, Shevchenko I, Umansky V, Nettelbeck DM, Weichert W et al (2014) CTLA-4 and PD-L1 checkpoint blockade enhances oncolytic measles virus therapy. Mol Ther 22(11):1949–1959
Hardcastle J, Mills L, Malo CS, Jin F, Kurokawa C, Geekiyanage H, Schroeder M, Sarkaria J, Johnson AJ, Galanis E (2017) Immunovirotherapy with measles virus strains in combination with anti-PD-1 antibody blockade enhances antitumor activity in glioblastoma treatment. Neuro Oncol 19(4):493–502
Gao Y, Whitaker-Dowling P, Griffin JA, Barmada MA, Bergman I (2009) Recombinant vesicular stomatitis virus targeted to Her2/neu combined with anti-CTLA4 antibody eliminates implanted mammary tumors. Cancer Gene Ther 16(1):44–52
Shen W, Patnaik MM, Ruiz A, Russell SJ, Peng KW (2016) Immunovirotherapy with vesicular stomatitis virus and PD-L1 blockade enhances therapeutic outcome in murine acute myeloid leukemia. Blood 127(11):1449–1458
Zamarin D, Holmgaard RB, Subudhi SK, Park JS, Mansour M, Palese P, Merghoub T, Wolchok JD, Allison JP (2014) Localized oncolytic virotherapy overcomes systemic tumor resistance to immune checkpoint blockade immunotherapy. Sci Transl Med 6(226):226ra232
Zamarin D, Ricca JM, Sadekova S, Oseledchyk A, Yu Y, Blumenschein WM, Wong J, Gigoux M, Merghoub T, Wolchok JD (2018) PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy. J Clin Invest 128(4):1413–1428
Quetglas JI, Labiano S, Aznar MA, Bolanos E, Azpilikueta A, Rodriguez I, Casales E, Sanchez-Paulete AR, Segura V, Smerdou C et al (2015) Virotherapy with a semliki forest virus-based vector encoding IL12 synergizes with PD-1/PD-L1 blockade. Cancer Immunol Res 3(5):449–454
Heinrich B, Goepfert K, Delic M, Galle PR, Moehler M (2013) Influence of the oncolytic parvovirus H-1, CTLA-4 antibody tremelimumab and cytostatic drugs on the human immune system in a human in vitro model of colorectal cancer cells. Oncol Targets Ther 6:1119–1127
Jenkins RW, Barbie DA, Flaherty KT (2018) Mechanisms of resistance to immune checkpoint inhibitors. Br J Cancer 118(1):9–16
Woller N, Gurlevik E, Fleischmann-Mundt B, Schumacher A, Knocke S, Kloos AM, Saborowski M, Geffers R, Manns MP, Wirth TC et al (2015) Viral infection of tumors overcomes resistance to PD-1-immunotherapy by broadening neoantigenome-directed T-cell responses. Mol Ther 23(10):1630–1640
CL drafted the original manuscript. Both CL and SW conceptualized and edited the manuscript. All authors read and approved the final manuscript.
We thank Indra Mahajan who assisted with proofreading and editing the manuscript.
The authors decalre that they have no competing interests.
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LaRocca, C.J., Warner, S.G. Oncolytic viruses and checkpoint inhibitors: combination therapy in clinical trials. Clin Trans Med 7, 35 (2018). https://doi.org/10.1186/s40169-018-0214-5
- Immune checkpoint inhibitors
- Oncolytic viral therapy
- Clinical trials
- Combination therapy