Schematic representation of proteomics platform applied in biomarker workflow. Initial discovery phase currently relies on untargeted MS-based approaches resulting in identification of vast number of potential biomarkers. Further verification requires targeted approach. Candidates should to be prioritized based on their functional/ biological relevance. Since the molecular changes underlying the pathological conditions are complex and heterogeneous, the ultimate solution to improve the accuracy of biomarkers appears to be the combination of biomarkers into a panel. The biomarker panel is evaluated in the verification step and further tested during the validation. Currently, immune-based approached are most commonly applied, although moderate selectivity of antibodies represents a significant problem. Alternatively, quantitative MS-based approach like MRM can be also introduced. Along with the advancements in biomarker workflow, the number of putative biomarkers is often decreasing, whereas the sample sets and general costs are increasing. In the validation phase, biomarker performance has to be assessed in a large cohort study in targeted population.