- Open Access
Managing the potential and pitfalls during clinical translation of emerging stem cell therapies
Clinical and Translational Medicine volume 3, Article number: 10 (2014)
We are moving into a new era of stem cell research where many possibilities for treatment of degenerative, chronic and/or fatal diseases and injuries are becoming primed for clinical trial. These reports have led millions of people worldwide to hope that regenerative medicine is about to revolutionise biomedicine: either through transplantation of cells grown in the laboratory, or by finding ways to stimulate a patient’s intrinsic stem cells to repair diseased and damaged organs. While major contributions of stem cells to drug discovery, safety and efficacy testing, as well as modelling ‘diseases in a dish’ are also expected, it is the in vivo use of stem cells that has captured the general public’s attention. However, public misconceptions of stem cell potential and applications can leave patients vulnerable to the influences of profit driven entities selling unproven treatments without solid scientific basis or appropriate clinical testing or follow up. This review provides a brief history of stem cell clinical translation together with an overview of the properties, potential, and current clinical application of various stem cell types. In doing so it presents a clearer picture of the inherent risks and opportunities associated with stem cell research translation, and thus offers a framework to help realise invested expectations more quickly, safely and effectively.
The immense scientific, media and public interest in stem cells over the past 15 years has largely been focused on two areas: the therapeutic potential of stem cells, and ethical controversies relating to the destruction of human embryos historically required to obtain pluripotent stem cells. What is rarely fully appreciated is the significant research progress made during these years that has resulted in a large number of stem cell-based clinical trials currently, or soon to be, underway[1, 2]. At the same time there has also been a rapid increase in the number and range of unproven stem cell therapies being offered worldwide. These unproven procedures are rarely underpinned by strong scientific evidence and are not tested through rigorous clinical trial assessment, thus making it difficult to support any claims made by the providers other than ‘selling hope’. Moreover, these unproven treatments are marketed directly to patients in an attempt to bypass the expert opinion of the patient’s treating physician(s). The emergence of unproven stem cell therapies alongside legitimate stem cell clinical trials neatly highlights the potential and pitfalls inherent within the stem cell field, both of which are driven by the desire of patients for desperately needed new treatments. Best management of these critically competing issues, to enable progress to be made as quickly as possible without exploitation of vulnerable patients, will be aided by a clear understanding of how the stem cell field has developed to where it is now.
Stem cell properties underpin their clinical potential
Stem cells can be divided into two broad categories, tissue-specific (‘adult’) stem cells and pluripotent stem cells. Both of these stem cell types are able to make identical copies of themselves during cell division, a process termed ‘self-renewal’. Importantly, as stem cells self-renew they retain the ability to produce the more mature functional cell types of the body, such as nerve, blood, heart or cartilage cells, through processes broadly termed ‘differentiation’. Tissue-specific stem cells normally only give rise to cells of the tissue in which they are found, for example, blood stem cells only produce blood cells. In contrast, pluripotent stem cells can produce any cell type of the body. In terms of clinical applications, both tissue-specific and pluripotent stem cells can be used to generate cells for autologous (patient-sourced) and allogenic (donor-sourced) therapies.
The development of clinical stem cell therapies has occurred progressively over the last 50 years, typically via the ‘medical innovations’ route (Figure 1). Commonly accepted clinical stem cell treatments are currently limited to therapies based on hematopoietic, skin, and corneal (limbal) stem cells. The first human hematopoietic transplant occurred in 1957 with the transplantation of bone marrow (containing blood stem cells) from one identical twin to the other in order to treat leukemia. This work ultimately led to the 1990 Nobel Prize in Physiology or Medicine being awarded to Drs Murray and Donnall Thomas. However, progress from this first experimental human transplant to routine clinical therapy took decades of painstaking and publicly-reported research into the molecular mechanisms of the hematopoietic system. This included: discovery of human leukocyte antigen matching and its importance in minimization of graft-vs-host disease (GVHD), and the discovery that hematopoietic stem cells (HSC) could be more easily obtained from peripheral blood (and subsequently cord blood) to provide less invasive and more efficacious transplant procedures. As a result of these concerted international research efforts over 50,000 life-saving HSC transplants are now routinely performed worldwide every year.
The success of HSC transplants led scientists and clinicians to research stem cell-based therapies for other tissues. In 1981 skin stem cells were first harvested from burns patients, amplified in the laboratory and then transplanted back to the patient to partially reconstitute their skin. The (limited) success of this initial procedure has led to a large number of skin grafts being performed since. However, more basic research is needed to improve the quality of the resulting skin so that it possesses more characteristics of normal skin such as sweat glands and hair follicles[9, 10].
In addition to saving lives, stem cell-based treatments have also been used to improve lives. In 1997 the first report was published showing that limbal stem or progenitor cells could be harvested from the edges of the cornea, proliferated in the laboratory and then transplanted back to patients to restore sight after corneal damage. While improved cell harvest methods, cell expansion methods, and transplantation substrates are needed, the current limbal stem cell approach is capable of restoring sight for some forms of corneal disease and injury (collectively termed limbal stem cell deficiencies). As a result, this therapy has become widely used particularly in developing countries where corneal injury is more prevalent[12, 13].
Arrival of new cell sources for stem cell therapies
To scientists, doctors, journalists, policy makers and the public alike it appears self-evident that a primary driver behind the development of the above three stem cell treatments was a desire to treat patients with fatal or chronically debilitating conditions. However, it has to be recognized that their refinement into effective clinical therapies, through dramatic improvements in efficacy and reduced treatment invasiveness, arose as a direct result of extensive basic research. This research, and its wide and transparent dissemination, created testable hypotheses that were assessed in controlled clinical settings. For example, the successful application of HSC for lymphopenia, leukemias and lymphomas only became possible after extensive research provided a detailed molecular understanding of GVHD[3, 4]. For skin and corneal grafts, targeted research into the identification, harvesting, expansion and transplantation of appropriate stem/progenitor cells was needed before the treatment of patients could be attempted[11, 14]. Clearly, successful development of these clinical therapies hinged on continuous evidence-based research to improve treatment efficacy and safety, and to reduce patient discomfort.
As a result of the clinical achievements made using hematopoietic, skin and corneal tissue-specific stem cells, tremendous international research efforts have been initiated to identify stem cells in other organs of the body. Tissue-specific stem cells have thus been identified in most organs, helping not only explain the regenerative capacities of the body but providing new opportunities for the development of stem cell-based treatments for diseases and injuries of these tissues (Table 1). In particular mesenchymal stem or stromal cells (MSC) identified in a wide range of tissues (including adipose tissue, bone marrow, amniotic fluid, umbilical cord, placenta, menstrual blood and dental pulp) have generated a great deal of clinical interest. MSC have been shown in vitro to differentiate into adipocytes, chondrocytes, osteoblasts, and myocytes[16, 17]. MSCs have also been shown to have immuno-modulatory properties that, dependant on the MSC source and treatment context, may demonstrate diverse effects from aiding tissue repair to promoting tumour formation.
Due to the ease of MSC harvest (e.g., via liposuction), as well as their differentiation and immunomodulation properties, a large number of MSC-based clinical trials are progressing based on both their potential for pharmaceutical action or cell-replacement[2, 20]. However, variations in protocols used for MSC purification, the lack of a definitive and universal MSC marker, and a poor molecular understanding of MSC action in disease settings currently make it difficult to interpret and compare much ‘MSC’ research and clinical data[21, 22]. Efforts to overcome these limitations are underway, such as the proposed use of minimal criteria for characterization of MSCs in order to improve both intra- and inter-laboratory data comparisons.
Autologous (patient-sourced) transplantation or in vivo activation of tissue-specific stem cells offers an opportunity to develop therapies without requiring immunomodulation. However, historic and continuing difficulties in purifying, maintaining and/or expanding tissue-specific stem cells in vitro has hampered development of novel therapeutic approaches using these cells. Moreover, the fact that tissue-specific stem cells can each only make a limited number of mature cell types makes production of complex tissues from these cells challenging. To overcome these significant practical limitations of tissue-specific stem cells, alternative sources of stem cells with greater developmental potential have been investigated. As a result, a variety of methods have been found for producing human pluripotent stem cells that are capable of generating any cell type in the body. These include: embryonal carcinoma cells derived from teratocarcinomas; embryonal germ cells; embryonic stem cells derived from donated supernumery preimplantation blastocysts or somatic cell nuclear transfer; and, perhaps most remarkably, induced pluripotent stem cells typically created by introducing expression of four exogenous ‘reprogramming factors’ into nucleated somatic cells. Importantly all of these human pluripotent stem cells, with the exception of embryonal carcinoma cells, can be maintained indefinitely in the laboratory with normal karyotype to provide a scalable source of normal (or diseased) human cells for research and clinical applications. The developmental potential, extensive proliferative ability, and economies of scale provided by human pluripotent stem cells confer enormous clinical potential to these cells (Table 1). However, the likely need for immunosuppression when transplanting their differentiated progeny provides a clear avenue for transplantation of autologous tissue-specific stem cells. As described in the next section, it remains to be seen which stem cell type (or types) will provide viable clinical therapies for particular diseases.
Clinical trial of emerging stem cell therapies
Key to developing any new cell therapy from tissue-specific or pluripotent stem cells is to identify, purify and in some cases expand the most appropriate stem or differentiated cell type (Figure 2). At the same time unwanted side-effects, such as transplantation of inappropriate or tumorigenic cells, need to be avoided. This requirement presents a common challenge for both tissue-specific and pluripotent stem cells since iterative experimentation is required to: i) identify the optimal cell type for disease intervention, and ii) establish appropriate conditions to generate and purify a clinically useful number of the required cell(s)[29–33]. A range of related considerations (and their influencing factors) that need to be addressed during development of stem cell-based clinical trials are listed in Table 2.
A recent review of the global landscape of stem cell clinical trials shows ‘novel’ applications - trials that do not involve established stem cell therapies - have grown dramatically, particularly using tissue-specific stem cells. Stem cell companies, hospitals and research institutions are supporting cell-based clinical trials for treatment of stroke, myocardial infarction, inflammatory bowel disease, GVHD, diabetes, critical limb ischemia, spinal cord injury and macular degeneration. Many different stem cell sources, including fetal tissue, umbilical cord, and adipose tissue are being investigated, with the majority of the trials currently underway involving MSCs from various sources. It should be noted, however, that due to the many unknowns surrounding MSC purification and mechanism described above, some leading stem cell clinician scientists have questioned the scientific basis and patient merits of some of these MSC trials[1, 22].
In contrast, the first clinical trial to be approved worldwide using cells derived from human pluripotent stem cells received approval from the USA Food and Drug Administration (FDA) in 2010. This Phase I trial used human embryonic stem cell-derived oligodendrocyte precursor cells to treat acute spinal cord injury. Unfortunately, despite the immense effort invested in navigating the FDA regulatory requirements to establish this ‘first in man’ trial, in 2011 it fell victim to the high costs involved. The company running the trial (Geron Corporation) made a controversial strategic decision to halt new enrolments, though the patients treated to this point continue to be monitored. This decision was made so the company could concentrate their resources on their anti-cancer platform. The decision came as a harsh blow to many researchers and patients who had been waiting for the opportunity to evaluate this new approach. It also raised important ethical questions such as the obligation to secure sufficient funds to complete a stem cell clinical trial.
Since commencement of the Geron trial two other human embryonic stem cell-based trials have begun and others are planned. The two commenced trials are using embryonic stem cell-derived retinal pigment epithelium to treat dry, age-related macular degeneration and Stargardt’s macular dystrophy. In 2013 the trial expanded from the USA to Europe, though it is unclear from the short-term patient data whether the transplanted cells have objectively improved visual acuity in the treated patients[35, 36]. Approval has also recently been obtained in Japan for the first clinical pilot study using retinal pigment epithelium derived from human induced pluripotent stem cells. Rather than using one donor cell source to treat multiple patients (as with the human embryonic stem cell-based trials), this induced pluripotent stem cell trial will provide patients with in vitro produced autologous cells. Six patients will have biopsies taken for induced pluripotent stem cell production, from which retinal pigment epithelial cells will be differentiated, purified and seeded onto a cell substrate for transplantation. This process will be time, labour and cost intensive, with it expected to take 10 months before the grafts are ready for transplantation. Given that human induced pluripotent stem cells were only first described in 2007 it is a remarkably short time to now be at the cusp of clinically testing these cells. However, it remains to be seen whether this type of highly technical and individualized patient-specific treatment is practical, effective and financially viable.
Unmet patient needs and the emergence of unproven commercial therapies
The complexity of many incurable conditions, together with our insufficient understanding of normal and abnormal biology, means that it will be years before many who hope to benefit from stem cell therapies will be able to participate in clinical trials. Moreover, while hundreds of registered stem cell-based clinical trials are currently underway for a wide range of conditions, the majority of these are in the early-phase of testing to establish the safety of the proposed interventions. As a result, the number of patients able to participate is limited and there are stringent exclusion criteria. These issues mean that many patients feel they are denied access to treatment. Rather than waiting to participate in clinical trials or waiting for the outcomes of these trials, many patients are prepared to risk pursuing unproven stem cell treatments outside of clinical trials and often without scientific basis, adequate safety data, or ethical and regulatory scrutiny[38–40]. Promoted on impressive-looking websites, these unproven therapies often make use of moving patient testimonials to attract patients but have very little in the way of evidence to support the claimed benefits[41, 42].
These unproven stem cell ‘treatments’ include the use of autologous and allogenic stem cell transplants, and are estimated to attract thousands of patients from around the world each year. Until recently, these unproven treatments have typically involved travel to countries with less stringent regulation of experimental stem cell treatments. Such travel is often dubbed ‘stem cell tourism’ and has raised great concern within the international stem cell community due to the potential for real harm that may befall patients undertaking the treatments. Indeed, complications including meningitis, encephalomyelitis, tumours[48, 49], aberrant bone formation and at least two reported deaths[51, 52] have all been attributed to unproven stem cell treatments.
More recently, there has been growth in the number of clinics selling treatments using a patient’s own cells in countries with considerable regulation such as Australia and the USA. Regularly but inaccurately marketed as having ‘no risks’, being ‘natural’, or being accepted medical practice, these unproven therapies routinely lack credible scientific evidence or overstate/inappropriately extrapolate the significance of animal data or preliminary human studies. In stark contrast to the ground-breaking but experimental hematopoietic and skin stem cell treatments that first occurred in the 1950s and 1980s respectively, these modern commercially-marketed unproven therapies typically have little or no patient follow-up and provide no data to further international research efforts into treatment development.
Proponents of unproven commercial therapies argue that a patient should have the right to pursue treatment using their own cells and blame over-regulation for delaying clinical progress of stem cell treatments[54, 55]. Even 2012 Nobel Prize winner in Physiology and Medicine, Sir John Gurdon, has criticised the FDA for placing "immense conditions on approval" saying "I think patients would be happy to take the risk of using their own cells given the choice". In contrast others are concerned that early adoption of unproven stem cell interventions, outside ethical review and the clinical trials processes, places individuals at risk of personal harm. Such unproven stem cell interventions also raise the significant risk of placebo effects being mistaken for genuine cures, and could stymie progress in the field. A recent statement from the International Society for Stem Cell Research (ISSCR) has urged ‘medical licensing bodies, legal authorities, patient advocacy organizations, physicians, and others to exercise their influence to discourage commercial provision of unproven autologous cell-based interventions outside of clinical trials’.
Evolving regulatory frameworks for stem cell therapies
Although there is still a role for assessment of innovative medical treatment using unproven stem cell interventions - like the initial 1957 hematopoietic and 1981 skin stem cell grafts - this approach should be considered as a ‘one-off’ and not offered widely or commercially until proven safe and effective. As recommended by the ISSCR this approach should only occur when certain conditions are satisfied such as: a robust scientific rationale; peer review of the proposed approach; data collection and clinical follow-up; undertaking only to treat a small number of patients who have provided informed consent; and commitment to more formal clinical trials in a timely manner (Figure 1).
Attempts to incorporate these recommendations while also decreasing the time for translating stem cell therapies are already underway. For example, recent legislation has provided the USA FDA with a new breakthrough therapy designation that offers the possibility of accelerated approval for investigational new drugs for serious conditions. In late 2013 Japan approved two new pieces of legislation: one that requires physicians to report the use of unapproved stem cell therapies to the health ministry, and another that creates a new approval channel for regenerative medicine and stem cell therapies[58, 59]. This new approval process appears similar to one used in South Korea, and also appears to be a variation of the currently accepted ‘medical innovations’ route of therapeutic development (Figure 1). At this stage though, a variety of important issues remain unclear such as: whether it will only be used to offer therapies to patients with no other viable alternatives; who will take responsibility for adverse outcomes; and what level of expertise will be required by the treatment provider.
This new Japanese legislation will enable companies to initiate small pilot treatment studies with as few as 10 patients without having to perform traditional Phase III clinical trials. If some degree of efficacy is shown in the pilot study, then the therapy can be approved for commercial use for 5 to 7 years, with possible coverage through the national health system. Once permission for commercialisation has been granted all patient data must be captured in a treatment registry, and approval can be withdrawn if therapies prove unsafe or ineffective.
While this new approach may reduce the risk of harmful side-effects from emerging and unproven stem cell therapies, it nevertheless has the potential to promote the sale of unproven and potentially costly stem cell therapies to desperate and vulnerable patients. It also raises the potential for patients to bypass currently available and effective treatments in favour of directly-marketed unproven therapies. It may also lead to misleading placebo effects becoming ingrained within the field since commercial treatments cannot be supplied, and their efficacy tested, as done with traditional double-blind clinical trials. Additionally, should any therapy commercialised in this way prove ineffective (or worse, identify previously unknown harmful side-effects), then large numbers of patients will have been subjected to inappropriate risks associated with medical procedures at significant health, financial and emotional cost.
The pace of research into tissue-specific and pluripotent stem cells continues to increase, with new advances announced regularly. In this review we see that solid foundations of basic molecular research are required, not necessarily for establishment of therapeutic concepts, but for development of therapies towards safe and effective clinical treatments. From less successful applications we see the dangers of unproven procedures and the challenges in developing therapeutic regimes with limited molecular understanding. As the appearance of stem cell-related clinical trials accelerates, failed trials and possibly unforeseen side-effects are likely to follow. At the same time there is a danger of harmful or fatal consequences resulting from the emergence of profit-driven unproven stem cell therapies that take advantage of vulnerable patients. A concerted effort is therefore required to identify pitfalls and safeguards for timely and effective clinical translation of emerging stem cell therapies. Additionally it is not yet clear which stem cell types will be best suited to treat particular diseases. Ultimately, only further empirical research will determine for each disease whether optimal treatment is provided by the extensive proliferative and differentiation potential of pluripotent stem cells, or the potential autologous nature of tissue-specific stem cells.
To address these issues we need to continue to diligently manage the expectation of patients and their families and friends, as well as those of the media, governments and financiers wanting to invest in the area. This includes correcting the misunderstanding that any source of stem cells is an automatic and unquestioned panacea for all ailments.
We need to accelerate access for patients to well-regulated and adequately funded clinical trials, but these trials need to be based on a detailed, mechanistic understanding of the disease process with strong supporting pre-clinical data. We must also acknowledge that it is going to take time to develop new stem cell treatments. This will help to avoid academic, social and economic pressures from eroding the integrity of, and community confidence in, the regenerative medicine field. The recent controversy surrounding the Stimulus-Triggered Activation of Pluripotency (STAP) method highlights this need, as well as the need for robust and transparent oversight processes at all levels, from basic research through to clinical translation.
We need to share results from all clinical research so that further research can be undertaken to evolve or develop promising new clinical therapies. We need to provide opportunities for innovative medicine on compassionate grounds but prevent over-enthusiasts from providing unproven and potentially unsafe procedures. Enforceable frameworks need to be developed that ensure new or ‘novel’ stem cell treatments are at least first proven safe through independent, peer-reviewed Phase I and II clinical trials in order to define for the patient the severity and frequency of unwanted side-effects.
We must ensure that any new therapy is provided only with informed patient consent and rigorous long-term post-treatment monitoring. We recommend that any commercially available stem cell therapies not assessed via Phase III clinical trials should be required to be designated as experimental treatments. It is only through recognising and addressing these issues that the field will safely progress at a rate closer to the expectations of patients, thus realising maximal patient benefits without simply ‘selling hope’.
Embryonic stem cells
Food and drug administration
Hematopoietic stem cells
International society for stem cell research
In vitro fertilization
Mesenchymal stem cell.
Daley GQ: The promise and perils of stem cell therapeutics. Cell Stem Cell 2012, 10: 740–749. 10.1016/j.stem.2012.05.010
Li MD, Atkins H, Bubela T: The global landscape of stem cell clinical trials. Regen Med 2014, 9(1):27–39. 10.2217/rme.13.80
Thomas ED, Lochte HL Jr, Lu WC, Ferrebee JW: Intravenous infusion of bone marrow in patients receiving radiation and chemotherapy. N Engl J Med 1957, 257: 491–496. 10.1056/NEJM195709122571102
Gatti RA, Meuwissen HJ, Allen HD, Hong R, Good RA: Immunological reconstitution of sex-linked lymphopenic immunological deficiency. Lancet 1968, 2: 1366–1369.
Singhal S, Powles R, Kulkarni S, Treleaven J, Sirohi B, Millar B, Shepherd V, Saso R, Rowland A, Long S, Cabral S, Horton C, Mehta J: Comparison of marrow and blood cell yields from the same donors in a double-blind, randomized study of allogeneic marrow vs blood stem cell transplantation. Bone Marrow Transplant 2000, 25: 501–505. 10.1038/sj.bmt.1702173
Gratwohl A, Baldomero H, Aljurf M, Pasquini MC, Bouzas LF, Yoshimi A, Szer J, Lipton J, Schwendener A, Gratwohl M, Frauendorfer K, Niederwieser D, Horowitz M, Kodera Y, Worldwide Network of Blood and Marrow Transplantation: Hematopoietic stem cell transplantation: a global perspective. JAMA 2010, 303: 1617–1624. 10.1001/jama.2010.491
O'Connor N, Mulliken J, Banks-Schlegel S, Kehinde O, Green H: Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1981, 1: 75–78.
Munster AM: Cultured skin for massive burns. A prospective, controlled trial. Ann Surg 1996, 224: 372–377. 10.1097/00000658-199609000-00013
Blais M, Parenteau-Bareil R, Cadau S, Berthod F: Concise review: tissue-engineered skin and nerve regeneration in burn treatment. Stem Cells Transl Med 2013, 2: 545–551. 10.5966/sctm.2012-0181
Snell JA, Loh NH, Mahambrey T, Shokrollahi K: Clinical review: the critical care management of the burn patient. Crit Care 2013, 17: 241. 10.1186/cc12706
Pellegrini G, Traverso CE, Franzi AT, Zingirian M, Cancedda R, De Luca M: Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997, 349: 990–993. 10.1016/S0140-6736(96)11188-0
Shortt AJ, Secker GA, Notara MD, Limb GA, Khaw PT, Tuft SJ, Daniels JT: Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results. Surv Ophthalmol 2007, 52: 483–502. 10.1016/j.survophthal.2007.06.013
Harkin DG, Apel AJ, Di Girolamo N, Watson S, Brown K, Daniell MD, McGhee JJ, McGhee CN: Current status and future prospects for cultured limbal tissue transplants in Australia and New Zealand. Clin Experiment Ophthalmol 2013, 41: 272–281.
Rheinwald JG, Green H: Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975, 6: 331–343. 10.1016/S0092-8674(75)80001-8
Lanza R, Gearhart J, Hogan B, Melton D, Pedersen R, Donnall Thomas E, Thomson J, Sir Ian W (Eds): Essentials of Stem Cell Biology. Second edition edition. San Diego: Elsevier Inc; 2009.
Uccelli A, Moretta L, Pistoia V: Mesenchymal stem cells in health and disease. Nat Rev Immunol 2008, 8: 726–736. 10.1038/nri2395
Ma S, Xie N, Li W, Yuan B, Shi Y, Wang Y: Immunobiology of mesenchymal stem cells. Cell Death and Differentiation 2014, 21(2):216–225. 10.1038/cdd.2013.158
English K, French A, Wood KJ: Mesenchymal stromal cells: facilitators of successful transplantation? Cell Stem Cell 2010, 7: 431–442. 10.1016/j.stem.2010.09.009
Houthuijzen JM, Daenen LG, Roodhart JM, Voest EE: The role of mesenchymal stem cells in anti-cancer drug resistance and tumour progression. Br J Cancer 2012, 106: 1901–1906. 10.1038/bjc.2012.201
Caplan AI, Correa D: The MSC: an injury drugstore. Cell Stem Cell 2011, 9: 11–15. 10.1016/j.stem.2011.06.008
Shen H: Stricter standards sought to curb stem-cell confusion. Nature 2013, 499: 389. 10.1038/499389a
Bianco P, Cao X, Frenette PS, Mao JJ, Robey PG, Simmons PJ, Wang CY: The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine. Nat Med 2013, 19: 35–42. 10.1038/nm.3028
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E: Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006, 8: 315–317. 10.1080/14653240600855905
Andrews PW, Matin MM, Bahrami AR, Damjanov I, Gokhale P, Draper JS: Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin. Biochem Soc Trans 2005, 33: 1526–1530. 10.1042/BST20051526
Shamblott MJ, Axelman J, Wang S, Bugg EM, Littlefield JW, Donovan PJ, Blumenthal PD, Huggins GR, Gearhart JD: Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc Natl Acad Sci U S A 1998, 95: 13726–13731. 10.1073/pnas.95.23.13726
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts. Science 1998, 282: 1145–1147.
Tachibana M, Amato P, Sparman M, Gutierrez NM, Tippner-Hedges R, Ma H, Kang E, Fulati A, Lee HS, Sritanaudomchai H, Masterson K, Larson J, Eaton D, Sadler-Fredd K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer RL, Wolf D, Mitalipov S: Human embryonic stem cells derived by somatic cell nuclear transfer. Cell 2013, 153: 1228–1238. 10.1016/j.cell.2013.05.006
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007, 131: 861–872. 10.1016/j.cell.2007.11.019
Irion S, Nostro MC, Kattman SJ, Keller GM: Directed differentiation of pluripotent stem cells: from developmental biology to therapeutic applications. Cold Spring Harb Symp Quant Biol 2008, 73: 101–110. 10.1101/sqb.2008.73.065
D'Amour KA, Bang AG, Eliazer S, Kelly OG, Agulnick AD, Smart NG, Moorman MA, Kroon E, Carpenter MK, Baetge EE: Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 2006, 24: 1392–1401. 10.1038/nbt1259
Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L: Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 2009, 27: 275–280. 10.1038/nbt.1529
Meyer JS, Shearer RL, Capowski EE, Wright LS, Wallace KA, McMillan EL, Zhang SC, Gamm DM: Modeling early retinal development with human embryonic and induced pluripotent stem cells. Proc Natl Acad Sci U S A 2009, 106: 16698–16703. 10.1073/pnas.0905245106
Osakada F, Jin ZB, Hirami Y, Ikeda H, Danjyo T, Watanabe K, Sasai Y, Takahashi M: In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J Cell Sci 2009, 122: 3169–3179. 10.1242/jcs.050393
Frantz S: Embryonic stem cell pioneer Geron exits field, cuts losses. Nat Biotechnol 2012, 30: 12–13. 10.1038/nbt0112-12
Schwartz SD, Hubschman JP, Heilwell G, Franco-Cardenas V, Pan CK, Ostrick RM, Mickunas E, Gay R, Klimanskaya I, Lanza R: Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet 2012, 379: 713–720. 10.1016/S0140-6736(12)60028-2
Huang J, McAlinden C, Wang Q, Jin ZB, Pesudovs K: Embryonic stem-cell-derived retinal pigment epithelial cells for macular degeneration. Lancet 2012, 379: 2050. author reply 2050–2051 author reply 2050–2051 10.1016/S0140-6736(12)60890-3
Japan to start stem-cell study on humans [http://blogs.nature.com/news/2013/07/japan-to-start-stem-cell-study-on-humans.html]
Lau D, Ogbogu U, Taylor B, Stafinski T, Menon D, Caulfield T: Stem cell clinics online: the direct-to-consumer portrayal of stem cell medicine. Cell Stem Cell 2008, 3: 591–594. 10.1016/j.stem.2008.11.001
Petersen A, Seear K, Munsie M: Therapeutic journeys: the hopeful travails of stem cell tourists. Sociol Health Illn 2013.
Regenberg AC, Hutchinson LA, Schanker B, Mathews DJ: Medicine on the fringe: stem cell-based interventions in advance of evidence. Stem Cells 2009, 27: 2312–2319. 10.1002/stem.132
Ryan KA, Sanders AN, Wang DD, Levine AD: Tracking the rise of stem cell tourism. Regen Med 2010, 5: 27–33. 10.2217/rme.09.70
Petersen A, Seear K: Technologies of hope: techniques of the online advertising of stem cell treatments. New Genetics and Society 2011, 30: 329–346. 10.1080/14636778.2011.592003
Zarzeczny A, Caulfield T: Stem cell tourism and doctors' duties to minors–a view from Canada. Am J Bioeth 2010, 10: 3–15.
Kiatpongsan S, Sipp D: Medicine. Monitoring and regulating offshore stem cell clinics. Science 2009, 323: 1564–1565. 10.1126/science.1168451
Hyun I, Lindvall O, Ahrlund-Richter L, Cattaneo E, Cavazzana-Calvo M, Cossu G, De Luca M, Fox IJ, Gerstle C, Goldstein RA, Hermerén G, High KA, Kim HO, Lee HP, Levy-Lahad E, Li L, Lo B, Marshak DR, McNab A, Munsie M, Nakauchi H, Rao M, Rooke HM, Valles CS, Srivastava A, Sugarman J, Taylor PL, Veiga A, Wong AL, Zoloth L, et al.: New ISSCR guidelines underscore major principles for responsible translational stem cell research. Cell Stem Cell 2008, 3: 607–609. 10.1016/j.stem.2008.11.009
Dobkin BH, Curt A, Guest J: Cellular transplants in China: observational study from the largest human experiment in chronic spinal cord injury. Neurorehabil Neural Repair 2006, 20: 5–13. 10.1177/1545968305284675
Alderazi YJ, Coons SW, Chapman K: Catastrophic demyelinating encephalomyelitis after intrathecal and intravenous stem cell transplantation in a patient with multiple sclerosis. J Child Neurol 2012, 27: 632–635. 10.1177/0883073811422831
Amariglio N, Hirshberg A, Scheithauer BW, Cohen Y, Loewenthal R, Trakhtenbrot L, Paz N, Koren-Michowitz M, Waldman D, Leider-Trejo L, Toren A, Constantini S, Rechavi G: Donor-derived brain tumor following neural stem cell transplantation in an ataxia telangiectasia patient. PLoS Med 2009, 6: e1000029.
Thirabanjasak D, Tantiwongse K, Thorner PS: Angiomyeloproliferative lesions following autologous stem cell therapy. J Am Soc Nephrol 2010, 21: 1218–1222. 10.1681/ASN.2009111156
In the Flesh: The Embedded Dangers of Untested Stem Cell Cosmetics [http://www.scientificamerican.com/article.cfm?id=stem-cell-cosmetics]
Europe’s largest stem cell clinic shut down after death of baby [http://www.telegraph.co.uk/news/worldnews/europe/germany/8500233/Europes-largest-stem-cell-clinic-shut-down-after-death-of-baby.html]
Croydon man died in long haul op, inquest hears [http://www.yourlocalguardian.co.uk/news/local/topstories/9491936.Man_died_in_long_haul_op/]
DeFrancesco L: Adult stem cell therapies walk the line. Nat Biotechnol 2012, 30: 739–741. 10.1038/nbt.2321
Sherman RE, Li J, Shapley S, Robb M, Woodcock J: Expediting drug development–the FDA's new "breakthrough therapy" designation. N Engl J Med 2013, 369: 1877–1880. 10.1056/NEJMp1311439
The FDA wants to regulate your cells [http://online.wsj.com/news/articles/SB10000872396390444405804577558992030043820]
Medicine Nobel: good choice, but will cures come soon? [http://www.newscientist.com/article/dn22354-medicine-nobel-good-choice-but-will-cures-come-soon.html#.UrZrQo24hPM]
ISSCR Statement of Delivery of Unproven Autologous Cell-based Interventions to Patients [http://www.isscr.org/home/about-us/news-press-releases/2013/2013/09/12/isscr-statement-of-delivery-of-unproven-autologous-cell-based-interventions-to-patients]
Seven days: 22–28 November 2013. The week in Science. Policy: Stem-cell laws [http://www.nature.com/news/seven-days-22–28-November-2013–1.14242#/policy]
Cyranoski D: Japan to offer fast-track approval path for stem cell therapies. Nat Med 2013, 19: 510. 10.1038/nm0513-510
Stem-cell scientist found guilty of misconduct [http://www.nature.com/news/stem-cell-scientist-found-guilty-of-misconduct-1.14974]
Permission to use Figure1 was supplied by the National Stem Cell Foundation of Australia. MM receives funding from the Australian Research Council as part of Stem Cells Australia. MO’C receives funding from the University of Western Sydney.
The authors declare that they have no competing interests.
The manuscript was conceived by MO’C with valuable input from MM and HM. HM, MM and MO’C all contributed to writing and editing the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Main, H., Munsie, M. & O’Connor, M.D. Managing the potential and pitfalls during clinical translation of emerging stem cell therapies. Clin Trans Med 3, 10 (2014). https://doi.org/10.1186/2001-1326-3-10
- Pluripotent stem cell
- Tissue specific stem cell
- Clinical trial
- Unproven treatment
- Regenerative medicine