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Clinical and Translational Medicine
Fig. 2 | Clinical and Translational Medicine

Fig. 2

From: Viral antigens detectable in CSF exosomes from patients with retrovirus associated neurologic disease: functional role of exosomes

Fig. 2

HTLV-1 infected cell lines have exosomes containing HTLV-1 Tax protein. a Western blots for levels of HTLV-1 Tax, Alix and β-actin (Actin) proteins were performed from cell lysates and NT80 + 82 nanotrapped exosomes from HUT102 and C8166 (C81) cells. Jurkat cell supernatants were also nanotrapped with NT80 + 82 particles and run alongside infected cell NT pulldowns. Tax levels detected were normalized to the β-actin signal for each sample and quantified in the upper right inset. b HTLV-1 Tax was measured by electrochemical ELISA. Nanotrapped (NT80 + 82) exosomes from Jurkat (Ju), C8166 (C81), and HUT102 (HT) cells were freeze-thawed and then measured for HTLV-1 Tax reactivity. Statistical significance was determined by unpaired t-test. ****p-value < 0.0001 (n = 4)

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