Using single-cell multiple omics approaches to resolve tumor heterogeneity

Clinical and Translational Medicine

Table 1 Notable advancements in single-cell techniques

Year introduced	Notable technology advancements	Method cell range^a
2009	Tang et al. [10]	1^b
2011	STRT-seq [23]	< 100
2012	SMART-seq [24]	< 100
2012	CEL-Seq [25]	< 100
2013	Fluidigm C1 (IFC) [26]	< 800
2013	Smart-seq 2 [27]	< 1000
2014	MARS-seq [28]	10,000 s
2015	Drop-seq [29]	10,000 s
2015	inDrop [30]	10,000 s
2016	Chromium (10× Genomics) [31]	10,000 s
2017	ddSeq (Bio-Rad) [32]	10,000 s
2017	SPLiT-seq [33]	10,000 s
2017	Seq-well [34]	10,000 s

This is a non-comprehensive list of peer-reviewed studies that advanced single-cell isolation and preparation techniques
^aThe “range” lists the largest relative population that can or has been studied using this technique
^bThis method involves mechanical separation and isolation of individual blastomeres into single wells