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Clinical and Translational Medicine
Fig. 6 | Clinical and Translational Medicine

Fig. 6

From: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

Fig. 6

Effects of Ca2+/hypoxia on the stem cell phenotypes of hUCB-MSCs. a Immunophenotypic analysis of CD34, CD45, CD73, and CD105 expression in naïve or Ca2+/hypoxia-conditioned hUCB-MSCs was performed using flow cytometry. b After incubation in specialized induction media, chondrogenic differentiation was measured by the accumulation of sulfated proteoglycan using safranin O staining (top), and osteogenic differentiation was evaluated by alkaline phosphatase staining (bottom). Data represent the mean ± SD (n = 3). *P < 0.05 versus the control as determined by Student’s t-tests. c The therapeutic effects of hUCB-MSCs in an elastase-induced emphysema model. Representative histological image of the lung parenchyma stained with hematoxylin-eosin (H&E). d Morphometric analysis of the mean linear intercept. Data represent the mean ± SD, n = 3; *P < 0.0001. Scale bar = 100 μm (magnification: 100 ×)

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