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Clinical and Translational Medicine
Fig. 1 | Clinical and Translational Medicine

Fig. 1

From: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

Fig. 1

Effects of Ca2+/hypoxia on the proliferation capacity of hUCB-MSCs. a, b hUCB-MSCs were treated with Ca2+, hypoxia, or Ca2+/hypoxia for 5 days. The morphology and growth curve of cultured hUCB-MSCs were analyzed. Ca2+/Hyp: Ca2+ plus hypoxia; Hyp: hypoxia. c hUCB-MSCs were treated with the indicated concentrations of Ca2+ under hypoxic conditions (3% oxygen) for 5 days. d Six batches of hUCB-MSCs were tested for proliferation in the Ca2+/hypoxia condition. The initial cell concentration of hUCB-MSCs was 5 × 103 cells/cm2. Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bar = 100 μm (magnification: 100 ×)

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