Fig. 1

C-Jun phosphorylation in HDM-challenged mice and the impact of CC-930 or vehicle control. a Schematic depicting the time course of HDM exposure and CC-930 administration. For instillation, 50 µg HDM or PBS as the vehicle control was administered intranasally once for 5 days over 3 weeks at the days indicated via the top arrows. Vehicle control (0.5 % CMC/0.25 % Tween 80) or CC-930 (100 mg/kg) was administered twice daily on the days indicated via the bottom arrows. Mice were euthanized 72 h after the last challenge. b Lung tissue was homogenized from PBS or HDM-challenged mice for assessment of phospho-c-Jun. Equal amounts of protein (20 μg for phosho-c-jun, 5 μg for actin) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and subjected to Western blot analysis for phospho-c-Jun and actin. Band intensity was determined and expressed as a ratio of phosphorylation to total protein (actin). Data shown represent mean ± SEM from two independent experiments (PBS: n = 10; HDM alone: n = 10; HDM + vehicle control: n = 11; HDM + CC-930: n = 11). *P < 0.05 (analysis of variance) versus PBS. ^† P < 0.05 versus the HDM group