Fig. 4

Factors affecting immunotherapeutic efficacy of anti-HER-2 × HBsAg. (a) SK-BR-3 , MCF-7, and the patients’ HER-2⁺ breast cancer cells were treated with trastuzumab, biosimilar anti-HER-2, anti-HBV, and anti-HER-2 × HBsAg in erythrocyte-free blood, in which concentrations of the complement system were adjusted according to measuring of C1q and C3 at 37 °C. The experiments were concluded by labeling of the cells with propidium iodide. Necrotic cells were counted by flow cytometry. The experiments were repeated three times. Increasing concentrations of complement system components resulted in increased efficacy of the breast cancer cells killing. The novel clusters anti-HER-2₀₀₄ × HBsAg and anti-HER-2₀₀₁ × HBsAg more than doubled the efficacy of the CDC over trastuzumab and anti-HER-2 biosimilars. b SK-BR-3, MCF-7, and the patients' breast cancer cells were treated with trastuzumab, anti-HER-2, anti-HBV, and anti-HER-2 × HBsAg is erythrocyte-free blood, in which the number of the immune cells was adjusted in relation to the number of breast cancer cells. Measurements were pursued as in (a). Increasing the ratio of the effector immune cells to target cancer cells resulted in proportional increase in efficacy of the breast cancer cells’ killing. The novel clusters anti-HER-2₀₀₄ × HBsAg and anti-HER-2₀₀₁ × HBsAg more than tripled the efficacy of the ADCC over trastuzumab and anti-HER-2 biosimilars