Fig. 12
PGE2 induces MT of HPMCs and MPMCs. Serum-starved HPMCs were treated with PBS, PGE2 (1 μM ) and TGF-β (5 ng/ml) for 48 h. Cell lysates were cleared, measured and then resolved by SDS-PAGE. a α-SMA was increased in TGF-β and PGE2-treated HPMCs. β-actin was the loading control. Image is representative of three independent experiments. b MPMCs were serum starved for 24 h and then treated with PBS, TGF-β (5 ng/ml) or PGE2 (1 μM) for 48 h. Lysates were then resolved via SDS-PAGE and probed for α-SMA. Akt was the loading control. Image is representative of two independent experiments. HPMCs were seeded on glass coverslips. Cells were serum starved for 24 h and then treated with PBS, PGE2 or TGF-β for 48 h. c PGE2 increased α-SMA expression by HPMCs and induced changes in cell morphology indicative of MesoMT. Images were taken at 40× and are representative of 30 fields/treatment. d Serum-starved HPMCs were treated with PBS, TGF-β (5 ng/ml) and sulprostone (EP3 agonist, 1 µM) for 48 h. Cell lysates were cleared, measured and then resolved by SDS-PAGE. α-SMA was increased in TGF-β and sulprostone-treated HPMCs. β-actin was the loading control. Image is representative of three independent experiments