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Clinical and Translational Medicine
Fig. 11 | Clinical and Translational Medicine

Fig. 11

From: Organizing empyema induced in mice by Streptococcus pneumoniae: effects of plasminogen activator inhibitor-1 deficiency

Fig. 11

COX-2 and PGE2 are induced in human and murine PMCs by plasmin and uPA. a Serum-starved HPMCs were treated with plasmin (PLN, 7 nM) or uPA (20 nM) for 48 h. Lysates were then Western blotted for COX-2. Akt was the loading control. b Conditioned media of cells treated with PBS, plasmin and uPA were analyzed for PGE2. Plasmin and uPA significantly increased PGE2 expression in HPMCs (* denotes a p < 0.05 when compared to PBS treated controls. n = 3/treatment). c Serum-starved MPMCs were treated with murine plasmin (7 nM, PLN) and murine uPA (20 nM) for 48 h. Lysates were then subjected to western blotting for COX-2. β-actin was the loading control. d Conditioned media of cells treated with PBS, plasmin and uPA were probed for PGE2 by competitive ELISA. Plasmin and uPA significantly increased PGE2 expression in MPMCs (*denotes a p < 0.05 when compared to PBS treated controls)

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