Figure 1From: Clinical potentials of human pluripotent stem cells in lung diseasesDiagram of hiPSC differentiation. The hiPSCs were cultured on matrigel-coated plates in DM containing 20 μg/ml of G418 for 14 days. The SPC positive cells were observed in the differentiating cultures at a very early time point (day 5), and can be enriched into a homogenous population (>99%) with robust proliferation capacity on day 10. The hiPSC-derived ATIICs were observed in the cultures on day 14, which expressed TTF1 and surfactant proteins (SPA, SPB, and SPC).Back to article page