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Fig. 4 | Clinical and Translational Medicine

Fig. 4

From: PI3K inhibitor treatment ameliorates the glucocorticoid insensitivity of PBMCs in severe asthma

Fig. 4

Establishment of the H2O2-TNFα-U937 cell model to mimic the dexamethasone insensitivity of PBMCs in severe asthma. a IL-8 mRNA expression in cells treated with H2O2, TNFα + H2O2, TNFα + DEX, or TNFα + H2O2 + DEX was measured by qPCR. b The IL-8 concentration in cells treated with H2O2, TNFα + H2O2, TNFα + DEX, or TNFα + H2O2 + DEX was measured by ELISA (p < 0.05, statistically significant. Each experiment was repeated at least three times)

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