From: The dual functional role of MicroRNA-18a (miR-18a) in cancer development
Target gene | Full name | Verification methods | Cell lines | Biological roles | References |
---|---|---|---|---|---|
IRF2 | Interferon regulatory factor 2 | Luciferase reporter assay | BEAS-2B, A549, HEK-293T | Promotes apoptosis, inhibits cell proliferation and migration | [54] |
SOX6 | Sex-determining region Y (SRY)-box 6 | Luciferase reporter assay, Western blotting | CaSki, ME-180, SiHa, PaCa-2 | Inhibits the Wnt/β-catenin pathway | [56] |
WNK2 | WNK lysine deficient protein kinase 2 | Luciferase reporter assay, Western blotting | CaSki, ME-180, SiHa, PaCa-2 | Inhibits the MEK/ERK pathway | [56] |
PTEN | Phosphatase and tensin homolog | Luciferase reporter assay, Western blotting | CaSki, ME180, SiHa, PaCa-2 | Inhibits the PI3K/AKT pathway | [56] |
STK4 | Serine/threonine-protein kinase 4 | Luciferase reporter assay, qRT-PCR | HEK-293T, DU145 | Promotes apoptosis, inhibits endogenous AKT activity | [58] |
PIAS3 | Protein inhibitor of activated STAT3 (signal transducer and activator of transcription 3) | Immunoprecipitation assay, qRT-PCR | MKN28, MKN1 | Inhibits STAT3 activity, downstream of the Wnt/β-catenin pathway | |
CDC42 | Cell division control protein 42 | Luciferase reporter assay | HCT116 | Acts as a GTPase in the PI3K/AKT pathway | [68] |
SREBP1 | Sterol regulatory element-binding transcription protein 1 | Luciferase reporter assay, Transwell assay, qRT-PCR | MDA-MB-231, MCF-7 | Promotes EMT and cell migration and invasion | [69] |
CTGF | Connective tissue growth factor | Luciferase reporter assay, Western blotting, qRT-PCR | 3T3 | Promotes cell proliferation, phosphorylation of ERK1/2 and AKT, crosstalk with the TGF-β pathway | [75] |
Nedd9 | Neural precursor cell expressed, developmentally downregulated 9 | Luciferase reporter assay, Western blotting, qRT-PCR | 3T3 | Downstream of the Wnt/β-catenin pathway, activates the MEK/ERK pathway | [75] |