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Table 1 List of experimentally validated miR-18a targets and their biological roles

From: The dual functional role of MicroRNA-18a (miR-18a) in cancer development

Target gene

Full name

Verification methods

Cell lines

Biological roles

References

IRF2

Interferon regulatory factor 2

Luciferase reporter assay

BEAS-2B, A549, HEK-293T

Promotes apoptosis, inhibits cell proliferation and migration

[54]

SOX6

Sex-determining region Y (SRY)-box 6

Luciferase reporter assay, Western blotting

CaSki, ME-180, SiHa, PaCa-2

Inhibits the Wnt/β-catenin pathway

[56]

WNK2

WNK lysine deficient protein kinase 2

Luciferase reporter assay, Western blotting

CaSki, ME-180, SiHa, PaCa-2

Inhibits the MEK/ERK pathway

[56]

PTEN

Phosphatase and tensin homolog

Luciferase reporter assay, Western blotting

CaSki, ME180, SiHa, PaCa-2

Inhibits the PI3K/AKT pathway

[56]

STK4

Serine/threonine-protein kinase 4

Luciferase reporter assay, qRT-PCR

HEK-293T, DU145

Promotes apoptosis, inhibits endogenous AKT activity

[58]

PIAS3

Protein inhibitor of activated STAT3 (signal transducer and activator of transcription 3)

Immunoprecipitation assay, qRT-PCR

MKN28, MKN1

Inhibits STAT3 activity, downstream of the Wnt/β-catenin pathway

[61, 66]

CDC42

Cell division control protein 42

Luciferase reporter assay

HCT116

Acts as a GTPase in the PI3K/AKT pathway

[68]

SREBP1

Sterol regulatory element-binding transcription protein 1

Luciferase reporter assay, Transwell assay, qRT-PCR

MDA-MB-231, MCF-7

Promotes EMT and cell migration and invasion

[69]

CTGF

Connective tissue growth factor

Luciferase reporter assay, Western blotting, qRT-PCR

3T3

Promotes cell proliferation, phosphorylation of ERK1/2 and AKT, crosstalk with the TGF-β pathway

[75]

Nedd9

Neural precursor cell expressed, developmentally downregulated 9

Luciferase reporter assay, Western blotting, qRT-PCR

3T3

Downstream of the Wnt/β-catenin pathway, activates the MEK/ERK pathway

[75]