From fiction to science: clinical potentials and regulatory considerations of gene editing

Clinical and Translational Medicine

Table 1 Summary of characteristics of different gene editing systems

	CRISPR	ZFN	TALEN	Meganuclease
Binding principle	RNA-DNA	Protein–DNA	Protein–DNA	Protein–DNA
Feasibility	Easy	Difficult	Difficult	Difficult
Construction	20 nucleotide sgRNA sequence per target site	Engineering new proteins for every target site	Engineering new proteins for every target site	Engineering new proteins for every target site
Ease of multiplexing	Easy	Difficult	Difficult	Difficult