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Table 1 Summary of characteristics of different gene editing systems

From: From fiction to science: clinical potentials and regulatory considerations of gene editing

  CRISPR ZFN TALEN Meganuclease
Binding principle RNA-DNA Protein–DNA Protein–DNA Protein–DNA
Feasibility Easy Difficult Difficult Difficult
Construction 20 nucleotide sgRNA sequence per target site Engineering new proteins for every target site Engineering new proteins for every target site Engineering new proteins for every target site
Ease of multiplexing Easy Difficult Difficult Difficult