Circulating monocytes and tumor-associated macrophages express recombined immunoglobulins in glioblastoma patients

Clinical and Translational Medicine

Table 1 Shared immunoglobulin heavy and light chain CDR3 variants in monocytes and TAM from 15 glioblastoma patients

CDR3	GBM001		GBM002		GBM003		GBM004		GBM005		GBM006		GBM0007		GBM008		GBM009		GBM010		GBM011		GBM012		GBM013		GBM014		GBM015
CDR3	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood	TAM	Blood
Shared immunoglobulin heavy chains
VRSWAYSLTPAGTGTYYFES		X	X
GRGGTGYYYYMDV			X	X
ARGAGVRYFDWLSPAGY			X		X	X
ARVPINYDILTGTDY					X	X
ARDEKQLVRNYYYYYGMDV																X	X							X
AKTLMVRGVRNAFDI																							X		X	X
AGEGSVVLTTSTFDI																							X	X						X
ARDWRSGGSCYYY																							X	X
Shared immunoglobulin light chains
HQSSNLPWT	X			X
IRVVIYLGR	X			X									X	X		X			X			X
MQGTHWPPTWT	X	X		X			X								X
QQYYRIPCT		X	X
QHYNNWPWT									X											X		X
QHYNDWPWT									X									X
QHYNAWPPWT										X								X
NTIMPGLRGR										X								X
QQSYSTPLT																X										X
QAWDSSTAV																											X	X

Shown are the shared immunoglobulin heavy (n = 8) and light chain (n = 10) CDR3 variants of monocytes and TAM from 15 glioblastoma patients. The CDR3 sequences were marked with an “X” in the respective cell preparations. Shared CDR3 variants were found between different glioblastoma patients (inter-individual sharing) and between the monocyte and TAM preparations of single glioblastoma patients (intra-individual sharing)