Fig. 4

Immunoglobulin repertoire features of macrophages and B cells. a The immunoglobulin heavy (left) and light chain (right) CDR3-length distributions are shown for the investigated tumor-associated macrophages and B cells from three representative patients. The number of nucleotides of the respective CDR3 sequence length (nt, x-axis) is plotted against the frequency of the respective CDR3 sequence length (n, y-axis). n = total number; nt = nucleotide. b The V_H- and J_H-family gene usage by TAM and B cells are indicated. The frequency (in %) of V_H family genes (left) and J_H-family genes (right) expressed by TAM and B cells, respectively, from three representative glioblastoma patients demonstrate a similar genes usages in both cell groups. c Distribution of the expressed V-chain genes of tumor-associated macrophages and B cells from three representative glioblastoma patients on the immunoglobulin heavy chain locus of chromosome 14. Here, macrophages show a restricted distribution with focus in the center of the locus compared to B cells which express more widely distributed V-chains. The distribution of all V chain genes expressed by TAM analyzed in this study is shown in Additional file 1: Figure S7