Fig. 1

Purity of the isolated CD14-positive cells validated by flow cytometry and gene expression profiling. a Flow cytometry of freshly isolated MACS purified CD14⁺ monocytes of one representatively chosen blood sample demonstrating purity of routinely 99.9% and absence of CD19⁺/CD22⁺ B cells. The analyses of PBMCs and CD19⁺ B cells are shown as positive controls for the used antibodies. b RT-PCR profiling is shown for CD14⁺ monocytes from one representative healthy individual. Expression of GAPDH, B cell (CD19), T cell (CD2) and monocyte/macrophage marker genes (CD14, CD68, CD163) demonstrates the quality of the isolated cell preparations and the absence of detectable quantities of B or T cells. PBMC and CD19⁺ B cells are demonstrated as reference. GAPDH glycerinaldehyd-3-phosphat-dehydrogenase, PBMC peripheral blood mononuclear cells