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Fig. 4 | Clinical and Translational Medicine

Fig. 4

From: Impact of fatty acid binding protein 5-deficiency on COPD exacerbations and cigarette smoke-induced inflammatory response to bacterial infection

Fig. 4

Restoration of FABP5 in alveolar macrophages is sufficient to reduce inflammation following CS and infection. FABP5−/− mice were inoculated with FABP5 lentiviral vector (Lenti-FABP5) or empty vector (Lenti-Control). Two weeks later, mice were exposed to 5 days of CS and subsequently infected with P. aeruginosa. Mice were harvested 16 h post infection. a Immunostaining for FABP5 (green) and CD68 (red) in the lung tissue of FABP5−/− mice inoculated with Lenti-Control or Lenti-FABP5. Bars represent 20 µm. b Total leukocytes, macrophages and neutrophils in the bronchoalveolar lavage (BAL) of FABP5−/− mice inoculated with Lenti-Control or Lenti-FABP5. Bars represent 50 µm. c IL-6 and KC protein levels in the BAL of FABP5−/− mice inoculated with Lenti-Control or Lenti-FABP5. d Hematoxylin and eosin stained paraffin sections of lungs from FABP5−/− mice inoculated with Lenti-Control or Lenti-FABP5. Bars represent 100 µm. e Lung tissue histopathology score of FABP5−/− mice inoculated with Lenti-Control or Lenti-FABP5. Data are representative of three independent experiments and represent the mean ± SEM. n = 5–6 mice/group. *p < 0.05, **p < 0.01

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