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Fig. 6 | Clinical and Translational Medicine

Fig. 6

From: Viral antigens detectable in CSF exosomes from patients with retrovirus associated neurologic disease: functional role of exosomes

Fig. 6

HAM/TSP exosomes can sensitize targets to Ag-specific responses. a Cytotoxic T lymphocyte (CTL) lysis was analyzed after the addition of HAM/TSP or ND PBMC-derived exosomes or Tax11-19 peptide. b HAM/TSP or ND PBMC-derived exosomes or Tax11-19 peptide pulsed targets were co-cultured with anti-HLA Class I ab (MCA81EL 5 μg/mL) and CTL target lysis was analyzed. c HAM/TSP PBMC supernatants were either nanotrapped with NT80 + 82 (NT) or with a control nanotrap (NT86) unable to isolate exosomes (ctrl). Supernatants collected after day 1, day 3, or day 5 of culture were used for trapping. d Various amounts (1 mL, 500, and 250 μL) of 5 days HAM 25 PBMC tissue culture supernatants were used as the starting material for nanotrapping by NT80 + 82 particles (NT), followed by CTL assay. e Eight representative HAM/TSP patient PBMC-derived exosomes were utilized for additional analysis of CTL lysis by CTL assay

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