Alk5/Runx1 signaling mediated by extracellular vesicles promotes vascular repair in acute respiratory distress syndrome

Clinical and Translational Medicine

Table 2 Expression of Runx1 isoforms in EV_ARDS and ARDS outcome

ARDS sample	Runx1^p66 expression	p66/p52 ratio	ICU length of stay (days	ARDS outcome
S2; S4; S5; S6; S10; S13; S14; S17; S18; S25; S26, S28; S31; S32; S33; S34; S35*;	Yes	High	28	14 long-term survivors 3 deceased*
S11; S12; S15	Yes	Low (< 0.5)	51	All deceased
S3; S9; S19; S23; S27;	No	–	12.4	All alive (the endogenous lung ability to repair is sufficient for recovery)
S7; S8; S20; S22	No	–	14.5	All deceased (the endogenous lung ability to repair not sufficient for recovery and no Runx1^p66 expression)

29 EV_ARDS preparations and 3 EV_Ctrl were lysed and subjected to WB analyses with Runx1 Ab. Runx1 immunoreactivity was quantified by densitometry (NIH ImageJ) and used to establish the Runx1p66/p52 ratio. EV_ARDS samples S5*, S14* and S35* illustrate special situations of three ARDS patients that died despite their high Runx1p66/p52 ratio due to other complications (S5) or removal of life support at family’s request (S14, S35)