Fig. 3
EVARDSAlk5 sub-population is derived from the MSCs. Unstained EVs were used as controls to determine fluorescence background as well as for gating purposes. a Representative forward and side scatter plot for unstained EVs. Background fluorescence in the PE and APC channels for the unstained samples shown as dot plot (b) and individual histograms (c, d). Similar, representative plot of EVs stained with Alk5-APC/CD105-PE Abs (f) to analyze the co-expression of these markers on the EVARDS (e, Gate D), together with the individual histograms for CD105-PE (g) and Alk5-APC (h). Representative plot of EVs stained with Alk5-APC/CD73-PE Abs (j) to analyze the co-expression of these markers on the EVARDS (i, Gate D), along with the histograms for CD73-PE (k) and Alk5-APC (l) are also shown. n = 9 EVARDS and 5 EVCtrl isolates. m Representative immunoisolation of CD105+ EVARDS Alk5 via biotin-conjugated CD105 Ab and streptavidin magnetic beads incubation. Lysates of EVARDS, bound and unbound EVARDS fractions were analyzed by SDS PAGE and WB. No immunoreactivity to CD45 and CD34 is detected. n = 6 EVs isolates/experimental condition, in three independent experiments