Fig. 1

Human ARDS lung tissue is deficient of ITSN and shows limited EC proliferation. a, b Representative ITSN-Alexa Fluor 594/CD31-Alexa Fluor 488 IHC of lung sections of ARDS and ND-Ctrl specimens, respectively; insets are higher magnifications of boxed areas. The arrowheads in a indicate a lung vessel with perivascular edema. Arrows in a, inset indicate the limited co-localization ITSN/CD31. Bars: 60 μm (a, b); 100 μm (insets). c Total protein extracted from ND-Ctrl and ARDS frozen lung tissues was analyzed by SDS PAGE (50 μg/lane) and WB with ITSN Ab. Actin served as loading control. The graph in c shows the average of ITSN/Actin ratio ± SD as determined by densitometric analyses of representative blots. *p < 0.01 vs. ND-Ctrl. n = 3. d Representative Ki67-Alexa Fluor 594/CD31-Alexa Fluor 488 IHC of lung sections of ND-Ctrl, and 2 ARDS specimens, a 55 year old male (e), and a 65 year old male (f); the number of days in ICU are indicated. Arrows in d indicate the co-localization CD31/Ki67. Sepsis was the cause of ARDS. Five ARDS and three ND-Ctrl samples were analyzed. Bar: 50 μm. n = 3 experiments