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Clinical and Translational Medicine
Fig. 6 | Clinical and Translational Medicine

Fig. 6

From: Lack of durable protection against cotton smoke-induced acute lung injury in sheep by nebulized single chain urokinase plasminogen activator or tissue plasminogen activator

Fig. 6

Levels of active PAI-1 in the BAL fluids of uninjured animals and animals with ISIALI. PAI-1 activity in BAL fluids of uninjured (naive; uninjured, not mechanically ventilated sheep, n = 3; sham; uninjured but mechanically ventilated sheep n = 2) animals and controls without treatment from Group 1 (GR1; n = 5) and Group 2 (GR2; n = 7) was measured using active rat PAI-1 ELISA (Molecular Innovations, Novi, MI; circles) and by titrating BAL fluid with uPA of a known concentration (squares) as previously described [15, 16]. The level of active PAI-1 in naïve (n = 3) and Group 1 sham (n = 2, not shown) was below level of detection using rat PAI-1 ELISA. Data are presented as box plots (showing median and interquartile ranges). There was no statistically significant difference between levels of active PAI-1 in the Group 1 versus 2 animals versus controls, although a trend towards increased BAL PAI-1 activity in sheep with ISIALI was observed

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