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Clinical and Translational Medicine
Fig. 5 | Clinical and Translational Medicine

Fig. 5

From: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

Fig. 5

Effects of Ca2+/hypoxia on the senescence of hUCB-MSCs. hMSCs were treated with Ca2+/hypoxia for five passages in the absence or presence of U0126 or BAPTA-AM. a Senescence stage was determined using SA-β-gal activity assays. b Staining was quantified by positive cell counts. c, d The mRNA levels of senescence-associated genes, i.e., p16, p53, and p21, or stemness marker genes, i.e., Oct4 and Nanog, were determined by real-time PCR analysis. e, f The expression levels of senescence-associated proteins, i.e., p21 and p-p53, were determined by western blot analysis. g Ca2+/hypoxia-induced cell proliferation was significantly decreased in the groups treated with U0126 or BAPTA-AM. Data represent the mean ± SD, n = 3; #P < 0.01, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bar = 100 μm (magnification: 100 ×)

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