Skip to main content
Fig. 3 | Clinical and Translational Medicine

Fig. 3

From: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

Fig. 3

Ca2+/hypoxia stimulated the proliferation of hUCB-MSCs via the HIF-1α-dependent pathway. a At 48 h post-transfection with control siRNA or HIF-1α-specific siRNA (si-HIF-1α), hMSCs were treated with Ca2+/hypoxia for 6 h. The expression of HIF-1α was analyzed by western blot analysis. b After growth under the Ca2+/hypoxia condition for 5 days, the proliferation capacity of HIF-1α-knockdown cells was measured by cell counting assays. c hUCB-MSCs were transfected with HIF-1α siRNA, pretreated with vehicle or 5 μM BAPTA-AM for 30 min, and then treated with Ca2+/hypoxia for 5 days. The proliferation capacity was determined by cell counting assays. Data represent the mean ± SD, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page