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Clinical and Translational Medicine
Fig. 2 | Clinical and Translational Medicine

Fig. 2

From: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

Fig. 2

Ca2+/hypoxia-treated hUCB-MSCs maintained their proliferation capacity in long-term culture. a, b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c, d After isolation of MNCs from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca2+/hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 103 cells/cm2. Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; *P < 0.05 versus control

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