From: Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis
Method | Reverse-transcription enzyme used | WTA method | Reverse-transcript size | Position bias |
---|---|---|---|---|
Tang’s method | Reverse transcriptase | Poly-A tailing | 0.5–3.0 kb | 3′-end |
Smart-seq/Smart-seq2 | M-MLV RT | Template-switching; locked nucleic acid in Smart-seq2 | Full-length | Low 3′-end |
Quartz-seq | Reverse transcriptase | Poly-A tailing; suppression PCR | 0.4–4.0 kb | 3′-end |
CEL-seq/CEL-seq2 | In vitro transcription | Poly-A tailing; barcoding | 3′-end only | High 3′-end |
STRT | Reverse transcriptase | Template-switching; barcoding | Full-length, only detect 5′-end | 5′-end |