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Table 2 Comparison of single-cell transcriptome sequencing methods.

From: Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis

Method

Reverse-transcription enzyme used

WTA method

Reverse-transcript size

Position bias

Tang’s method

Reverse transcriptase

Poly-A tailing

0.5–3.0 kb

3′-end

Smart-seq/Smart-seq2

M-MLV RT

Template-switching; locked nucleic acid in Smart-seq2

Full-length

Low 3′-end

Quartz-seq

Reverse transcriptase

Poly-A tailing; suppression PCR

0.4–4.0 kb

3′-end

CEL-seq/CEL-seq2

In vitro transcription

Poly-A tailing; barcoding

3′-end only

High 3′-end

STRT

Reverse transcriptase

Template-switching; barcoding

Full-length, only detect 5′-end

5′-end

  1. WTA whole-transcriptome amplification, SMART switching mechanism at the 5′-end of RNA template, M-MLV RT Moloney murine leukemia virus reverse transcriptase, CEL-Seq cell expression by linear amplification and sequencing, STRT single-cell tagged reverse transcription sequencing