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Table 1 Comparison of whole-genome amplification methods for single-cell DNA sequencing.

From: Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis

Method Enzyme used Application Genome coverage SNV detection CNV detection Amplification bias
DOP-PCR Taq DNA polymerase Single nucleus sequencing Low (~10%) High false negative and false positive rates Useful High (102–106 fold)
MDA φ29 DNA polymerase; Bst DNA polymerase Single nucleus exome sequencing Moderate (>70%) Useful but has a high false negative rate due to amplification bias Not accurate Moderate (3- to 4-fold)
MALBAC Bst DNA polymerase Single-cell genome/exome sequencing High (>90%) High false positive rate due to low fidelity Accurate Low
  1. SNV single nucleotide variant, CNV copy number variant, DOP-PCR degenerate oligonucleotide-primed polymerase chain reaction, MDA multiple-displacement amplification, MALBAC multiple annealing and looping based amplification cycles