From: Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis
Method | Enzyme used | Application | Genome coverage | SNV detection | CNV detection | Amplification bias |
---|---|---|---|---|---|---|
DOP-PCR | Taq DNA polymerase | Single nucleus sequencing | Low (~10%) | High false negative and false positive rates | Useful | High (102–106 fold) |
MDA | φ29 DNA polymerase; Bst DNA polymerase | Single nucleus exome sequencing | Moderate (>70%) | Useful but has a high false negative rate due to amplification bias | Not accurate | Moderate (3- to 4-fold) |
MALBAC | Bst DNA polymerase | Single-cell genome/exome sequencing | High (>90%) | High false positive rate due to low fidelity | Accurate | Low |