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Fig. 3 | Clinical and Translational Medicine

Fig. 3

From: Novel paradigm for immunotherapy of ovarian cancer by engaging prophylactic immunity against hepatitis B virus

Fig. 3

Sensitivity and specificity of AVEC: anti-HER-2 × HBsAg to attract anti-HBV antibodies. The ovarian cancer cells from cultures (OV90, TOV112, SK-OV-3) and from the patients (001-0012), human ovary surface (HOSE), human artery endothelial (HAE) cells were labeled initially with: (a) trastuzumab followed by the anti-FcR-BD fluorescent antibody, (b) trastuzumab followed by anti-HBsAg fluorescent antibody, (c) AVEC: anti-HER-2 × HBsAg followed by the anti-FcR-BD fluorescent antibody, (d) AVEC: anti-HER-2 × HBsAg followed by anti-HBsAg fluorescent antibody. The assays were repeated four times. The data presented are representative for all acquired. These cells revealed having high number of HER-2 receptors (>2 × 106/cell) were effectively labeled with both trastuzumab and AVEC (a, c). However, only the cells labeled with AVEC attracted the anti-HBsAg antibody onto the ovarian cancer cells (d), but the cells labeled with trastuzumab did not (b). (e) Blood from the patients (V-HBV-001-003) vaccinated against HBV were depleted of erythrocytes, magnetic AVEC’s: anti-HER-2 × HBsAg were used to pull out anti-HBsAg, while the concentrations were adjusted to 10.0 mIU/ml or as in those administered in the forthcoming experiments, and electrophoresed. The gels were quantified. These data show very specific and sensitive affinity of the patients’ anti-HBsAg towards AVEC. Some of the antibodies were undergoing some degree of chain separation showing up as faster bands. (f) Blood from the patients vaccinated against HBV were depleted of erythrocytes, magnetic AVEC’s: anti-HER-2 × HBsAg were used to pull out anti-HBsAg and electrophoresed without adjusting the concentrations. The gels were quantified. The quantifications revealed different concentrations of the anti-HBsAg in different patients and different degree dissociation of the chains. The assays were repeated four times. The data presented are representative for all acquired

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