Fig. 2From: Integrated EpCAM-independent subtraction enrichment and iFISH strategies to detect and classify disseminated and circulating tumors cellsComparison of EpCAM expression among different types of cancer cell. IF staining of EpCAM was performed on adenocarcinoma cells of colon (SW480), pancreas (PANC-1) and NSCLC (A549). SW480 cells show very high expression of EpCAM on the plasma membrane. Most of PANC-1cells have very low amount of EpCAM localized in cytoplasm and vesicles, and one out of five cells shows EpCAM in nucleus (green arrow). All of A549 cells, except two negative in the middle (white arrows), have weak nucleus staining of EpCAM. Plasma membrane localization of EpCAM is not visualized on both PANC-1 and A549 cellsBack to article page