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Fig. 6 | Clinical and Translational Medicine

Fig. 6

From: Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma

Fig. 6

Inhibition of MDM2 by nutlin-3 restored wild type p53’s apoptotic response: Since PLK-1 also indirectly controls p53’s activity via MDM2, we determined the effect of PLK-1 inhibition on MDM2 functioning. a Inhibition of PLK-1 with BI-2536 did not change the levels of the MDM2 protein. b Quantitation of the MDM2 protein western after BI-2536 treatment expressed relative to β-actin. c Treating the cells with nutlin-3, an inhibitor of MDM2, reduced the viability of both the H295R and SW-13 ACC cell lines. d Furthermore, inhibition of MDM2 restored wild type p53’s apoptotic response in H295R cells as determined by the Caspase 3/7 glo assay. e However, a very small increase in apoptosis was observed in the SW-13 cells with mutant p53. Doxorubicin was used as a positive control for apoptosis. All experiments were done at least three individual times and data are represented as means with standard error. Uncropped blots that include molecular weight standards can be found in the Additional file 1: Figure S3

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