Fig. 2

Inhibition of Polo-like kinase 1 (PLK-1) reduced the viability of ACC cell lines: Given that PLK-1 is a negative regulator of p53, inhibition of PLK-1 should reduce viability of the ACC cell lines. a ACC cell lines, H295R and SW-13 were treated with PLK-1 siRNA and amount of PLK-1 protein was determined after 72 h of siRNA treatment. b Quantitation of PLK-1 protein western after siRNA knockdown expressed relative to β-actin. c Knocking down expression of the PLK-1 protein reduced viability of the H295R & SW-13 cell lines as compared to the controls. d, e Similarly, treating the cells with BI-2536, an inhibitor of PLK-1, reduced the viability of both the H295R and SW-13 ACC cell lines. Assays were normalized first to cells alone and then to a vehicle control. This resulted in viability measures of greater than 100 %. All experiments were done at least three individual times and data are represented as means with standard error. TF Transfection agent and NT Non-targeting siRNA. Uncropped images of full blots with molecular weight markers can be found in the Additional file 1: Figure S1