Molecular analysis of KIAA1549-BRAF fusion genes in LGG. A, Schematic representation of KIAA1549-BRAF gene fusions. Red dotted line represent common fusion sites within intron 15–16 and 16–17 of KIAA1549 gene and 8–9, 10–11 in BRAF introns. Red dotted arrows represent 15–11 primer used in screening LGA samples. B, Identification of different gene fusions with RT-PCR using 15_F-11_R primer set showing three different product sizes corresponding to 16–9, 15–9 and 16–11 gene fusions. C, Partial Sequence chromatogram profile generated from fusion transcript shown in B confirming the fusion between 16–9, 15–9 and 16–11. Arrows indicate the junction between KIAA1549 and BRAF genes.