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Figure 3 | Clinical and Translational Medicine

Figure 3

From: Gene expression profiling of immunomagnetically separated cells directly from stabilized whole blood for multicenter clinical trials

Figure 3

Cells are not activated by experimental procedure. (A) Four experimental groups (EG) were designed to test whether whole blood cells are activated at time of blood draw and transfer to PAXgene Blood RNA tubes (EG 1), and after storage of blood in EDTA tubes for 20 minutes prior to transfer to PAXgene Blood RNA tubes (EG2). Additionally, whole blood cells, which had been exposed to EDTA, were incubated with CD14ab-coated magnetic beads (EG3) or without magnetic beads (EG4). The perturbation score is nearly constant for all conditions, indicating a lack of activation. Mean values per group and the standard error of mean are shown. (B) Separated cells were stored in EDTA for up to 7 days prior to sorting. The perturbation score level is different for each cell type, but does not change significantly per cell type, indicating that cells are not activated during the experiment including the sorting process. Mean values per group and the standard error of mean are shown. (C) Perturbation score analysis for CD14+ cells which had been stored in EDTA as room temperature (RT). The score increases after 4 hours storage time. The experiment was discontinued after 24 hours.

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