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Figure 2 | Clinical and Translational Medicine

Figure 2

From: Gene expression profiling of immunomagnetically separated cells directly from stabilized whole blood for multicenter clinical trials

Figure 2

RNA integrity and yield over time. (A) The influence of formaldehyde (HCHO)-based and EDTA-based additives on RNA integrity, when whole blood was stored at ambient temperature or at 4°C for up to 7 days prior to sorting. EDTA-admixtures resulted in constantly high RIN values for the cell preparations up to 7 days of storage at 4°C. (B) All five tested cell types (N = 5 each) showed a good total RNA quality when stored in the EDTA blood collection tubes at 4°C for up to 7 days prior to sorting. (C) Total RNA extraction for all five tested cell types (N = 5 each) resulted in total RNA amounts sufficient for further downstream experiments even when stored in the EDTA blood collection tubes at 4°C for up to 7 days. In all panels, mean values per group and the standard error of mean are shown.

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