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Figure 7 | Clinical and Translational Medicine

Figure 7

From: Transcriptional factor snail controls tumor neovascularization, growth and metastasis in mouse model of human ovarian carcinoma

Figure 7

H&E staining of necropsy lung for number of metastatic clusters per lung in paraffin-embedded tissues taken from xenograft tumor-bearing RAGxCγ double mutant mice. (A) Mice were implanted with 0.5×106 A2780, A2780 shRNA Slug and A2780 shRNA Snail cells cutaneously on the rear flank and OP treatment began daily (i.p.) 7 days post implantation when tumors reached 50 mm3. Paraffin-embedded tissue sections (5μm) on glass slides were processed for H&E staining for each mouse necropsied at indicated day post-implantation. Stained tissue sections were photographed using AxioCam MRc5 digital color camera attached to a Zeiss Imager M2 fluorescence microscope at 400× magnification. Images are representative of at least five fields of view from two tissue sections. (B) Metastatic lung clusters as representative in the insert were microscopically counted per tissues and plotted in the graph. Statistical analysis was carried out using GraphPad Prism and results were compared by unpaired t-test.

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