Figure 6From: Transcriptional factor snail controls tumor neovascularization, growth and metastasis in mouse model of human ovarian carcinomaA2780, A2780 shRNA Snail and A2780 shRNA Slug ovarian cancer cells in heterotopic xenograft of tumors growing in RAGxCγ double mutant mice. (A) Cells at 0.5×106 in 0.2 mL were implanted cutaneously in the right back flank of these mice. Twice a week following implantation of the cancer cells each mouse was monitored for tumor volume ((width square/2) × length) at the site of implantation. Mice were sacrificed at day 24 post-implantation. (B) Representative tumors on the right flank of the animal, necropsy tumors and H&E staining of tumors; N/A, not available due to lack of tumor. (C) Paraffin-embedded tumor sections (5μm) on glass slides were processed for immunohistochemistry using primary DyLight 488 conjugated rat monoclonal anti-mouse CD31+ (PECAM-1) antibody in 1% BSA in PBS blocking solution and Entellan× rapid mounting media. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 400× magnification. Images are representative of at least five fields of view from two tumor sections.Back to article page