Skip to main content
Clinical and Translational Medicine
Figure 1 | Clinical and Translational Medicine

Figure 1

From: Transcriptional factor snail controls tumor neovascularization, growth and metastasis in mouse model of human ovarian carcinoma

Figure 1

EGF induces sialidase activity in live (A) A2780 ovarian cancer cells, (B) A2780 shRNA Snail and (C) A2780 shRNA Slug. Cells were allowed to adhere on 12 mm circular glass slides in media containing 10% fetal calf sera for 24 h. After removing media, 0.318 mM 4-MUNANA substrate (2′-(4-methlyumbelliferyl)-α-N-acetylneuraminic acid) in Tris buffered saline pH 7.4 was added to live cells alone (control) or with EGF alone and in combination with OP, anti-Neu1 neutralizing antibodies, and specific inhibitor of MMP-9i at the indicated dosage. Fluorescent images were taken at 1-2 min after adding substrate using Zeiss Imager M2 epi-fluorescence microscopy (20× objective). The mean fluorescence of 50 multi-point replicates was quantified using the Image J software. The data are a representation of one out of three independent experiments showing similar results.

Back to article page