Histopathological findings mean severity (Number affected/Total number of animals) | ||||
---|---|---|---|---|
4-week study | 8-week study | 12-week study | ||
Organ/tissue | MSC-NTF cells single injection | MSC-NTF cells 2 injections | MSC-NTF cells 3 injections | Vehicle 3 injections |
Males | ||||
Injection sites – thigh muscles | ||||
Blood vessel – inflammation subchronic, focal | 0.2 (1/5) | 0.0 (5/5) | 0.0 (5/5) | 0.0 (4/4) |
Myofiber subchronic inflammation, multifocal | 0.6 (2/5) | 1.0 (5/5) | 1.0 (5/5) | 0.0 (4/4) |
Presence of intracellular vacuoles at the site of inflammation | 0.0 (5/5) | 0.6 (3/5) | 0.8 (4/5) | 0.0 (4/4) |
Presence of multinucleated giant cells at the site of inflammation | 0.0 (5/5) | 0.2 (1/5) | 0.2 (1/5) | 0.0 (4/4) |
Interstitium subchronic inflammation, multifocal | 0.2 (1/5) | 0.0 (5/5) | 0.0 (5/5) | 0.0 (4/4) |
Interstitium –fibrosis, multifocal | 0.2 (1/5) | 0.0 (5/5) | 0.0 (5/5) | 0.0 (4/4) |
Females | ||||
Injection sites – thigh muscles | ||||
Myofiber subchronic inflammation, multifocal | 1/0 (5/5) | 1.0 (5/5) | 1.0 (5/5) | 0.0 (5/5) |
Presence of intracellular vacuoles at the site of inflammation | 0.8 (4/5) | 0.2 (1/5) | 0.8 (4/5) | 0.0 (5/5) |
Presence of multinucleated giant cells at the site of inflammation | 0.2 (1/5) | 0.2 (1/5) | 0.4 (2/5) | 0.0 (5/5) |
Granuloma-foreign body, focal | 0.4 (2/5) | 0.0 (5/5) | 0.0 (5/5) | 0.0 (5/5) |