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Table 6 Histopathological findings in male and female C57BL/6 J mice following single or repeated injections of MSC-NTF cells or the vehicle control

From: Safety of repeated transplantations of neurotrophic factors-secreting human mesenchymal stromal stem cells

Histopathological findings mean severity (Number affected/Total number of animals)

 

4-week study

8-week study

12-week study

Organ/tissue

MSC-NTF cells single injection

MSC-NTF cells 2 injections

MSC-NTF cells 3 injections

Vehicle 3 injections

Males

    

 Injection sites – thigh muscles

    

 Blood vessel – inflammation subchronic, focal

0.2 (1/5)

0.0 (5/5)

0.0 (5/5)

0.0 (4/4)

 Myofiber subchronic inflammation, multifocal

0.6 (2/5)

1.0 (5/5)

1.0 (5/5)

0.0 (4/4)

 Presence of intracellular vacuoles at the site of inflammation

0.0 (5/5)

0.6 (3/5)

0.8 (4/5)

0.0 (4/4)

 Presence of multinucleated giant cells at the site of inflammation

0.0 (5/5)

0.2 (1/5)

0.2 (1/5)

0.0 (4/4)

 Interstitium subchronic inflammation, multifocal

0.2 (1/5)

0.0 (5/5)

0.0 (5/5)

0.0 (4/4)

 Interstitium –fibrosis, multifocal

0.2 (1/5)

0.0 (5/5)

0.0 (5/5)

0.0 (4/4)

Females

    

 Injection sites – thigh muscles

    

 Myofiber subchronic inflammation, multifocal

1/0 (5/5)

1.0 (5/5)

1.0 (5/5)

0.0 (5/5)

 Presence of intracellular vacuoles at the site of inflammation

0.8 (4/5)

0.2 (1/5)

0.8 (4/5)

0.0 (5/5)

 Presence of multinucleated giant cells at the site of inflammation

0.2 (1/5)

0.2 (1/5)

0.4 (2/5)

0.0 (5/5)

 Granuloma-foreign body, focal

0.4 (2/5)

0.0 (5/5)

0.0 (5/5)

0.0 (5/5)