FKBP51 modulates the TGF-β signal in normal melanocytes. A, left Real-time PCR measurement of FKBP51, mRNAs in melanocytes, which were transfected with FKBP51 plasmid or the empty vector (EV) as control. The enhancement of FKBP51 transcript validated the efficacy of transfection. A, right Real-time PCR measurement of Cyclin B and CDH2 mRNAs in melanocytes, FKBP51- or EV-transfected, cultured in the absence or the presence of 10 ng/ml TGF-β for 3 days. The relative change of expression in different samples was estimated relative to EV sample (expression = 1). B, Phase contrast microscopy of melanocytes FKBP51- or EV-transfected, cultured in the absence or the presence of 10 ng/ml TGF-β for 3 days. Upper; EV- or FKBP51-melanocytes presented rounded/polygonal morphology. Lower left; EV- melanocytes cultured with TGF-β presented slender spindle shape with multiple dendritic extensions. Lower right; FKBP51-melanocytes cultured with TGF-β displayed elongated shape and bipolar spindle morphology.