Figure 4

FKBP51 enhances expression of pro oncogenic factors in A375 melanoma cells stimulated with TGF-β. A Normalized expression rates (mean±s. d.) of SLUG (upper), and FKBP51 (lower) mRNA levels. SH-NS sample expression=1 (N=2). RNA was extracted from the melanoma cell line A375 stably transfected with a specific FKBP51 shRNA (SH-FKBP51), or a non-silencing (SH-NS) shRNA as control, in the absence or the presence of 10 ng/ml TGF-β. After a 18 h culture, cell was harvested. B Western blot assay of vimentin expression in whole cell lysates prepared from SH-NS and SH-FKBP51 cells cultured in the absence or the presence of 10 ng/ml TGF-β for 18 h. TGF-β stimulated an increase in vimentin level in SH-NS but not SH-FKBP51 cells. Reduced basal levels of vimentin were observed in SH-FKBP51 cells.