Figure 3

FKBP51 enhances expression of pro oncogenic factors in SAN melanoma cells stimulated with TGF-β. A, upper Western blot assay of Sparc levels in cell lysates obtained from the melanoma cell line SAN transfected with a specific FKBP51 shRNA (SH-FKBP51), or a non-silencing (SH-NS) shRNA as control, in the absence or the presence of 10 ng/ml TGF-β. After a 18 h culture, cell was harvested for lysates preparation. A, lower Flow cytometric histograms of Vimentin expression in SH-FKBP51 or SH-NS, in the absence or the presence of 10 ng/ml TGF-β. Cell was harvested after a 18 h culture. Vimentin was measured by indirect immunofluorescence, in fixed and permeabilized cells. B Effect of FKBP51 siRNA on VIM and SLUG expression levels. Normalized expression rates (mean±s. d.) of VIM (upper), SLUG (intermediate) and FKBP51 (lower) mRNA levels. NS RNA-treated sample expression=1 (N=2). Melanoma cell line SAN was transfected with a specific FKBP51 siRNA, or a non-silencing (NS) RNA as control. After 24 h from transfection 10 ng/ml TGF-β was added to the cultures. Cell was harvested after further 18 h and total RNA was extracted.