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Clinical and Translational Medicine
Figure 2 | Clinical and Translational Medicine

Figure 2

From: A pilot study demonstrating a non-invasive method for the measurement of protein turnover in skin disorders: application to psoriasis

Figure 2

Protein identification of soluble and insoluble skin proteins. Prior to tape stripping, the skin surface was gently washed. The washes from psoriatic lesions contained abundant protein. Turnover rates of wash derived proteins were compared to tape strip proteins. The wash liquid was collected, centrifuged to separate soluble and insoluble fractions (tape strip processing removes soluble proteins), and processed using the same washing/SDS extraction process used for tape strips. a). Incorporation of deuterium from ingested heavy water in tape strips and in proteins from the wash. b). SDS PAGE stained with coomassie blue comparing the proteins recovered from a tape strip (middle lane) with the soluble and insoluble fractions from the skin surface wash of the same subject.

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