Bioinformatics insights into acute lung injury/acute respiratory distress syndrome

Clinical and Translational Medicine

Table 2 Examples of application for proteomics in the pathogenisis of ALI in 2009-2010

Protein	technologies		Ref.
caveolin-1, CD44, annexin A2, protein S100-A10, or filamin A/B	Quantitative proteomic analysis	HMW-HA, via CD44-mediated CEM signaling events, represents a potentially useful therapeutic agent for LPS induced increasing of lung vascular permeability	[52]
MMP-9		Administration of doxycycline might be a significant supportive therapeutic strategy in prevention of VILI.	[53]
MMP-3, TIMP-1	liquid chromatography- electrospray ionization mass spectrometry	MMPs in the BALF of premature neonates maybe related to BPD development	[54]
VEGFR1, VEGFR2, NRP-1	Immunohistochemistry	VEGFR I and VEGFR2 were significant up-regulated in early ARDS while Neuropilin-1 was down-regulated.	[55]
PAI-1	ELISA	Higher PAI-1 levels are associated with increased mortality and fewer ventilator-free days among pediatric patients with ALI.	[56]
RAGE, PCPIII, BNP, ANG2, IL10, TNF-[alpha], IL8	bead-based cytokine array, singleplex enzyme-linked immunosorbent assay	seven plasma biomarkers had a high diagnostic accuracy in differentiating patients with trauma-induced ALI	[57]
AFT3	Microarray analysis, ELISA	ATF3 may act to counterbalance CS and high volume induced inflammation in Ventilator-induced Lung Injury	[58]

HMW-HA:high-molecular-weight hyaluronan; CEM: caveolin-enriched microdomains; MMP:matrix metalloproteinase; TIMP:tissue inhibitors of metalloproteinases; VILI:ventilation-induced lung injury; BPD:bronchopulmonary dysplasia; GAPDH:glyceraldehyde-3-phosphate dehydrogenase; VEGF:vascular endothelial growth factor; NRP-1:neuropilin-1; RAGE:receptor for advanced glycation end products; PCPIII:procollagen peptide III; BNP:brain natriuretic peptide; ANG2:angiopoietin-2; IL-10:interleukin-10, TNF-alpha:tumor necrosis factor alpha; IL-8:interleukin-8.