Immunofluorescence analysis of the subcellular BCRP localization. Cells were treated with 2 μM of LY294002 for 12 h, collected as a cell block, formalin-fixed, paraffin-embedded, and processed. BCRP was detected as green fluorescence, while cell nuclei were indicated by purple fluorescence. Scale bar, 30 μm. Reproduced by permission from Spandidos Publications. Inc. Imai Y, et al. (19).