Application of a Minusheet® tissue carrier in compatible perfusion culture containers. (a) A first type of perfusion culture container can hold six tissue carriers to provide them with always fresh medium. (b) In a gradient perfusion culture container the respective tissue is exposed to different fluids at the luminal and basal side. (c) In a microscope container with a transparent lid and base developing tissue can be observed. (d) Perfusion culture set-up is running on a laboratory table. A thermo plate maintains the desired temperature of 37°C. During culture a peristaltic pump transports the medium (1.25 ml/h) from a storage bottle (left side) to the waste bottle (right side).