Figure 1
From: Functional phosphoproteomic mass spectrometry-based approaches
A possible proteomic work-flow for clinical studies. For identification, in-gel or in-solution proteins of interest are excised, washed and enzimatically digested to extract peptides for further analysis by MS. The mass to charge (m/z) ratio of the retained peptides can be measured by a mass spectrometer. Data-computer analysis is carried out which mainly includes: identification and classification of proteins, validation of identified protein, interpretation and classification of the protein lists, structural analysis of proteins, PTM analysis and Cellular composition.